3C Protease (GST Tag)

The PreScission Protease (HRV 3C Protease) produced by GeneUniversal Inc. was a human rhinovirus type 14 3C protease expressed in E. coli with GST tag, it could recognize specifically octapeptide sequence Leu-Glu-Val-Leu-Phe-Gln-Gly-Pro or core pentapeptide sequence Leu-Phe-Gln-Gly-Pro at low temperature (4℃), and digested between glutamine and glycine. It was often used for removing affinity tag, such as Glutathione S-transferase(GST)tag, 6×His tag or other affinity tag.

Storage Condition

It could be preserved for 2 years at -80℃, and it could be preserved for 6 months at -20℃. Suggest to subpackage into small volume yourself, and avoid repeated freeze-thaw cycles.

Operation Method

1. Made up the follow reaction system in EP tube:
Fusion protein:1mg
Prescission Protease:10U
2. Mixed up and then incubated at suitable temperature, suggest reaction at 4℃ overnight.
3. Analyzed by SDS-PAGE after reaction, and Prescission Protein could be removed by GST tag affinity chromatography.

Product Specifications

Product names Catalog Number Specification Price
PreScission Protease MC01050 50U $16
MC01250 250U $70
MC01500 500U $120

Related Services

To meet your needs to the fullest extent, Gene Universal also provides Oligo Synthesis,Gene Synthesis,Molecular Biology,Protein Expression.

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