Custom Gene Library

DNA libraries refer to a collection of large numbers of DNA sequences that have been cloned into vectors, researchers working in fields such as protein engineering, antibody engineering, enzyme engineering, synthetic biology, discovery biology, and structural biology can identify and isolate the DNA fragments interested for further study. Gene Universal offers a wide range of library types, include Site-directed Mutagenesis Library, Scanning Library, Randomized Library, Degenerated Library, and Truncation Library.

FEATURED SYNTHETIC DNA LIBRARIES

1.Site-directed Mutagenesis Library: Site-directed mutagenesis library means a customer-determined amino acid position will be substituted sequentially with all the other 19 amino acids to create a library of mutants. With this approach, scientist can use structural-functional analysis to examine the benefits or adverse effects of a particular amino acid substitution.

2.Scanning Library: Scanning Library means substituting some residues sequentially with one specified residue within a customer-determined region by site-directed mutagenesis. Alanine and Cysteine scanning are two of the most popular used scanning libraries.

3.Randomized Library: A randomized mutant library will allow you to create random amino acid substitutions in a small user-defined region (ie,ORF region within 200bp-1500bp). These proteins can then be expressed and assayed for function to determine beneficial substitutions in protein engineering studies.

4.Degenerated Library: By using different genetic elements in multiple combinations, scientist could create a library of mutants to help them find the most beneficial combination. Gene Universal offers both traditional partially randomized (NNK/NNS) and completely randomized (NNN) mutant library services

5.Truncation Library: A truncation library of mutants is created by trimming amino acids from either the N- or C-terminus (or both) of a protein around a specified core region. This will allow scientist to identify the core region responsible for protein function.

Mutant Type Capacity Quality Control Deliverable

Site-directed Mutagenesis Library

Individual, 100% sequence-verified clones

Certificate of Analysis (COA) :
1.Restriction digest map
2.Sequence trace data with alignment
3.Sequence files of the synthetic gene alone or subcloned into a vector

2-5 µg of lyophilized plasmid DNA

Scanning Library Individual, 100% sequence-verified clones

Certificate of Analysis (COA) :
1.Restriction digest map
2.Sequence trace data with alignment
3.Sequence files of the synthetic gene alone or subcloned into a vector

2-5 µg of lyophilized plasmid DNA
Randomized Library

Ready-to-clone PCR Fragment Library or Cloned Pooled Library Up to 109 variants

Sequence verification of a pre-determined number of clones (with statistical analysis) based on library size

PCR fragment Library: 2-4 µg of linear ds DNA (ready-to-clone via 5’ and 3’ restriction sites if desired)
Cloned pool Library:
1. 5-10 µg of lyophilized plasmid DNA
2.Glycerol stock of total library with up to 109 transformants

Combinatorial Library

Ready-to-clone PCR Fragment Library or Cloned Pooled Library Up to 109 variants

Sequence verification of a pre-determined number of clones (with statistical analysis) based on library size

PCR fragment Library: 2-4 µg of linear ds DNA (ready-to-clone via 5’ and 3’ restriction sites if desired)
Cloned pool Library:
1. 5-10 µg of lyophilized plasmid DNA
2.Glycerol stock of total library with up to 109 transformants

Truncation Library

Individual, 100% sequence-verified clones

Certificate of Analysis (COA) :
1.Restriction digest map
2.Sequence trace data with alignment
3.Sequence files of the synthetic gene alone or subcloned into a vector

2-5 µg of lyophilized plasmid DNA


If you have custom requests or require any pre-project consultation, please contact us at sales@geneuniversal.com

HOW TO ORDER?

Please describe your project in detail and attach all the relevant documents to sales@geneuniversal.com, our project manager will contact you within 12 hours.

Related Services

To meet your needs to the fullest extent, Gene Universal also provides Oligo Synthesis,Molecular Biology,Protein Expression.

Ordering Information

Or download and fill out the order form below and email to order@geneuniversal.com. Our sales representative will contact you within 24 hours.

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E-mail: sales@geneuniversal.com

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