Bacterial Expression

Gene Universal specializes in E.coli protein expression service with risk-free guarantee. Our Guaranteed Bacterial Recombinant Protein Expression Service Package consists of codon optimization service, followed by gene synthesis and subcloning, all the way through protein expression and purification. With the risk-free guarantee, it is no cost to you if project fails. The overall success rate is over 95% in E.coli expression systems. Place your order today to experience unprecedented, high quality services yet competitive pricing Bacterial Protein Expression Services.

Service Descriptions

  • Gene synthesis is included in the package(gene length less than 1500 bp).
  • With hundreds of different Bacterial Protein Expression vectors(ie. pET series, Psumo,pGEX) combined with multiple E.coli Strains ( ie. BL21, Origami DE3), Gene Universal provides flexible portfolio to meet your demand.
  • Risk-Free: No Protein, No Charge. If project fails, no cost to you.
  • Guaranteed Protein Quantity & Purity: no protein loss when bundling with endotoxin removal option ,no cost to you if desired purity & amount are not achieved.
  • Low Price yet Fast Turnaround: As low as $1450 for 3mg protein at 85%, in as fast as 4 weeks.
  • High Success Rate: Greater than 95% in E.coli expression system.
  • Customized: Find suitable combination of quantity, purity, His tag ,GST tag or tag-free, and endotoxin removal.

  • Customer Provides
    Tag
    Amount
    Timeline
    Purity
    Endotoxin Level Options
    Pricing
    Target protein sequence
    Small Tag(e.g. His)
    3mg
    4-6 weeks
    85%
    ≤1.0 EU/μg
    Starting from $1450
    ≤0.1 EU/μg
    90%
    ≤0.01 EU/μg
    ≤0.001 EU/μg

    Service Procedure

    Service Options

    Package Details:

    Steps
    Specification
    Timeline
    Deliverables
    Gene synthesis& Cloning, Plasmid Prep

    1.Codon optimization, Gene synthesis, Sub cloning into expression vector

    2.Plasmid Prep and DNA QC

    ≤2 weeks

    1. Protein in buffered solution with agreed amount & purity – Guaranteed

    2. Synthesized genes

    3. Optimized gene sequence report

    4. QC data

    Protein Expression Pilot

    1.Transform plasmids into appropriate bacterial expression strain

    2.Protein expression evaluation and optimization

    ≤1 week
    Protein Purification, Tag Removal (Optional) & Refolding (Optional)

    1. Scale up protein expression using optimized conditions

    2. Purification to reach desired protein amounts and purity

    3. Tag removal (if requested)

    4. Refolding in case of insoluble protein (performed only if required)

    ≤1 week
    QC & Delivery

    1. Purity analysis and Detection( SDS-PAGE and Western Blot-tagged protein only)

    2. Protein delivery

    ≤1 week



    Terms and Conditions:

    1.Protein sequences are subject to pre-project analysis.
    2.Exclusions are applicable to membrane proteins, toxic proteins and for target proteins greater than 800 amino acids.
    3.To increase the expression level of your protein, codon optimization using our proprietary is highly recommended.
    4.Insoluble proteins maybe subject to our proprietary protein refolding platform.

    Related Services

    To meet your needs to the fullest extent, Gene Universal also provides Oligo Synthesis,Gene Synthesis,Molecular Biology.

    Quote Now

    Ordering Information

    Or download and fill out the order form below and email to order@geneuniversal.com. Our sales representative will contact you within 24 hours.

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    Gene Universal Inc.

    Address: 200 Continental Dr. Suite 401, office 402, Newark DE 19713

    Tel:+1 302-235-9792

    E-mail: sales@geneuniversal.com

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