Insect Cell Expression

Compared with Bacterial Protein Expression ,there are several advantages of using baculovirus expression system, such as improved solubility, ability to incorporate post-translational modifications, and higher yields for secreted proteins. Using our baculovirus expression system we can establish the best possible protocol for your custom project and then scale up to get the highest expression of your target protein. With the stable experimental system, high expression level and post-translation modification, baculovirus expression is widely recognized as a powerful tool for expressing large-scale proteins.

Service Descriptions

  • Our baculovirus expression Services start with Gene Universals’codon optimization service, followed by gene synthesis and sub cloning, all the way through protein expression and purification using our bacterial protein expression system.
  • Gene Universal possesses the most advanced AKTA purifier system, along with the pre-positioned Ion column, molecular sieve, hydrophobic interaction column and affinity column.
  • Gene Universal’s Endotoxin Removal Service can effectively reduce the endotoxin level below 10 EU/mg during recombinant protein production with a success rate of 98%.
  • Service Procedure

    Service Options

    Bac-to-Bac system service type:
    No. Service type Working day Comment
    1 Gene synthesis and subcloning 10-15 pFastBac1, pFastBacHT….
    2

    Bacmid preparation ,transfection and expression evaluation

    14 PCR evaluation and P1 virus preparation
    3 P2 virus amplification 6 P2 virus amplification and MOI optimization
    4 Small scale 8 200ml expression and purification
    5 Largescale Enquiry Shaker flask/ bioreactor

    Terms and Conditions:

    1.We will start the project according to your choice and will only charge the cost step by step.
    2.Affinity purification, ion exchange purification, hydrophobic purification and SEC purification can be used. The expression system will affect the final protein purity and activity.

    Related Services

    To meet your needs to the fullest extent, Gene Universal also provides Oligo Synthesis,Gene Synthesis,Molecular Biology.

    Ordering Information

    Or download and fill out the order form below and email to order@geneuniversal.com. Our sales representative will contact you within 24 hours.

    • SEND A MESSAGE

    CONTACT US

    Gene Universal Inc.

    Address: 200 Continental Dr. Suite 401, office 402, Newark DE 19713

    Tel:+1 302-235-9792

    E-mail: sales@geneuniversal.com

    Copyright © 2017 Gene Universal, Inc.