Mammalian Expression

Mammalian cell expression system is a complex glycosylation protein expression system, transient expression in mammalian cells is especially crucial when post-translational modifications and appropriate protein folding is desired for downstream applications. It is most widely used in the development and production of recombinant protein drugs, especially for therapeutic recombinant monoclonal antibody.Gene Universal’s mammalian expression services offer rapid production and delivery of high quality proteins and recombinant antibodies (rAbs) from microgram to gram quantities in either CHO or HEK293 cells, with guaranteed protein amount and purity. We use the proprietary transient mammalian expression technology to modify cellular pathways to achieve optimal protein and antibody yields (up to 3 g/L).Our guaranteed mammalian protein expression packages start with gene synthesis and sub cloning, all the way through protein expression and purification using our proprietary mammalian protein expression & purification technologies.

Service Descriptions

  • One-Stop Solution-From gene to protein till antibody
  • Gene synthesis is included – no additional cost.
  • Competitive price-Cost-effective, exactly know how much you would scale-up based on the pilot study.
  • Fast turnaround time-as fast as 3 weeks.
  • Extremely low endotoxin level: as low as 1 EU/mg.
  • No setup fee.
  • Flexibility-Provide customized package according to your requirements.
  • Service Procedure


    Service Options

    Mammalian system service type:
    No. Service Type Working day
    1 20 mL Expi 293/CHO system expression and purification (Prepare endotoxin-free plasmids, Transfection,Expression and purification) 16
    2 100 mL Expi 293/CHO system expression and purification (Prepare endotoxin-free plasmids, Transfection,Expression and purification) 20
    3 1-50 L Expi 293/CHO system expression and purification (Prepare endotoxin-free plasmids, Transfection,Expression and purification) Inquire

    *Package applicable for secreted proteins and extracellular domain (ECD) of membrane proteins ≤150kDa.

    *QC data: SDS-PAGE will be performed to determine protein purity. BCA or Bradford assay will be used to measure protein concentration and A280 will be used to measure antibody fragment concentration.

    Terms and Conditions:

    1.We will start the project according to your choice and will only charge the cost step by step.
    2.Affinity purification, ion exchange purification, hydrophobic purification and SEC purification can be used. The expression system will affect the final protein purity and activity.

    Related Services

    To meet your needs to the fullest extent, Gene Universal also provides Oligo Synthesis,Gene Synthesis,Molecular Biology.

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    Ordering Information

    Or download and fill out the order form below and email to order@geneuniversal.com. Our sales representative will contact you within 24 hours.

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