Single-Stranded DNA Synthesis

Single-stranded DNA (ssDNA), with high editing efficiency and lower off-target integration, is thought to be the best CRISPR Homology Directed Repair (HDR) template for creating gene knock-in to develop transgenic animal models and cell lines. Gene Universal now offers high quality and sequence verified ssDNA for maximizing the editing efficiency of your CRISPR-mediated gene knock-in, in-vitro transcription, and more experiments.

Advantages of leveraging ssDNA as CRISPR-mediated gene knock-in HDR Templates.
  • Lower cellular toxicity
  • Reduced off-target integration
  • High efficiency
  • Increased editing accuracy

  • Service Features
  • Sequence verification by sanger sequencing
  • Non-detectable levels of dsDNA and minimum DNA base damage
  • Free gene template storage, supporting further re-orders
  • Expertise in synthesizing difficult genes as ssDNA templates
  • Rapid delivery – Industry-leading turnaround time
  • Yield flexibility – Choose 3, 5, 10 or even more µg of lyophilized fragments.

  • Length (Nucleotides) Quantity Price Turnaround Time (Business Days)
    151-500 3 ug $350 10-12 Business Days
    5 ug $500
    10 ug $750
    >10 ug Inquiry Inquiry
    501-3000 3 ug $0.75/nt 15-18 Business Days
    5 ug $0.9/nt
    10 ug $1.2/nt
    >10 ug Inquiry Inquiry
    3001-5000 Inquiry Inquiry Inquiry
    *Please note: Shipped as lyophilized powder, 50% off complementary sequence for every order, complex sequences (GC/AT rich, poly structure, hairpin, repeats) need to be quoted.


    1.CRISPR-mediated gene knock-in, replacement, or correction
  • Double Stranded Breaks (DSBs)
  • Guide RNA (gRNA)
  • Protospacer Adjacent Motif (PAM)
  • Non-Homologous End-joining (NHEJ)
  • Homology Directed Repair (HDR)

  • 2.In vitro transcription
    Transcription is the first of several steps of DNA based gene expression in which a particular segment of DNA is copied into RNA (especially mRNA) by the enzyme RNA polymerase. In vitro transcription (IVT) method means the transcription of RNA molecules, via RNA polymerase (T7, T3, or SP6) by using DNA templates in vitro. Several types of DNA templates could be used for IVT, including double-stranded DNA fragments, circular plasmid DNA, and single-stranded donor oligonucleotides (ssODN) or single-stranded single-stranded DNA (ssDNA).

    Other application areas:
    Antibody Discovery
    Cancer Biology
    Food technology

    Deliverables and Quality Control Documents:
  • Delivery format: Lyophilized.
  • Digested by S1 nuclease, dsDNA residues will be verified by agarose gel electrophoresis
  • Sequence verified via Sanger sequencing,
  • Purity tested by gel -electrophoresis, sterilized by 0.22um filter membrane, A260/A280=1.8-2.0

  • How to Order?
    Email to submit custom inquiries.

    Related Services

    To meet your needs to the fullest extent, Gene Universal also provides Oligo Synthesis,Molecular Biology,Protein Expression.

    Ordering Information

    Or download and fill out the order form below and email to Our sales representative will contact you within 24 hours.



    Gene Universal Inc.

    Address:200 Continental Dr. Suite 401, office 402, Newark DE 19713

    Tel:+1 302-235-9792


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