Recombinant Antibody

Gene Universal has extensive expertise and research experience in the field of recombinant antibody expression. We have successfully delivered more than 2,000+ recombinant proteins / antibodies. We can produce recombinant antibodies in a wide range of formats including IgG, IgA, IgM,scFv, Fab, sdAb, Fc fusion proteins etc, which are sure to meet your requirements. Simply provide us with a gene sequence, and we will take care of the rest.

Advantages of Recombinant Antibody:

1. High expression Expi293/CHO system, highest transfection yield could reach more than 1g/L;

2. Have variety of constant region vectors, such as hIgG1/hIgG4/mIgG2a etc, to improve antibody production efficiency;

3. Animal-free: Recombinant antibodies have the identical DNA and protein sequence, in vitro production can be implemented. This process does not require use of animals.

4. Faster: Using protein expression system, such as mammalian system, the production time of recombinant can be weeks compared to months for monoclonal antibodies.

5. Consistency and reproducibility: Production process is controlled and reliable. Meanwhile, this leads to antibodies with very little batch-to-batch variability, giving highly reproducible results.

Recombinant Antibody (rAb) Services Overview

Starting Material step Service Type Timeline Deliverables Price
Antibody Sequence (Minimum 3 samples) 1 Gene synthesis(variable region), Cloning into one Constant Vector, Plasmid prep 17-22 BD Plasmid,Clone strain,Antibody Medium and SDS-PAGE data $350 per antibody
(including gene synthesis)
Get a Quote
2 10ml H-T transfection (Expi293/CHO)
3 H-T purificaiton (Magnetic ProteinA beads 3 BD Plasmid, Clone strain, Purified antiboy and COA $250/Ab (Optional)
Starting Material step Service Type Timeline Deliverables Price
Antibody Sequence (Minimum 10 samples 1 Gene synthesis(variable region), Cloning into one Constant Vector, Plasmid prep 15-20 BD Plasmid,Clone strain,Antibody Medium and Biacore T200 concentration test report Starting at $350 per antibody (including gene synthesis) Get a Quote
2 3ml H-T transfection (Expi293/CHO)
3-1 Biacore T200 test expression medium Antibody concentration
3-2 H-T purificaiton (Magnetic ProteinA beads) 3 BD Plasmid, Clone strain, Purified antiboy and COA $150/Ab(Optional)
  • 1. Project can begin from gene synthesis or customer-provided plasmid but Antibody sequences are required for project evaluation in both cases.
  • 2. Turnaround time does NOT include shipping details.
  • 3. Pricing applicable to human IgG1, human IgG4, mouse IgG2a, and mouse/human chimeric with hIgGI/hIgG4; Kappa (ĸ)/lambda(Λ) light chains.
  • 4. Additional QC such as SEC-HPLC, Endotoxin Testing,is available upon request (pricing may vary).
  • 5. If ≥85% (antibody #) are expressed and delivered, the whole project will be charged in full; If <85% (antibody #) are expressed and delivered, we will charge for the delivered antibodies, and only charge a basic setup fee for gene synthesis, cloning and expression evaluation of the antibody sequences that are failed to deliver.
Service Type Timeline Price Deliverables
5mg 22 BD $1,500 PAGE Purity: >95%; Conc: >0.5 mg/mL; Endotoxin: <10 EU/mg; COA Get a Quote
10mg $1,800
20mg $2,300
50mg 30 BD $4,000 PAGE Purity: >95%; Conc: >1.0 mg/mL; Endotoxin: <10 EU/mg; COA Get a Quote
100mg $6,500
  • 1. Recombinant antibody expression starts from gene synthesis and cloning of the "Antibody Variable region sequences" provided by clients. All molecular biology work is included in the listing price.
  • 2. This price table applies to full length human IgG1 and IgG4 expression.
  • 3. Purity is determined by SDS-PAGE by default. SEC-HPLC is available upon request(Price may vary). A280 will be used to measure rAb concentration.
Service Type Timeline Deliverables Price
1-50L expression and purificaiton Quote SEC-HPLC Purity: >95%;Endotoxin: <1 EU/mg;COA Quote
Service Type Timeline Deliverables Price
CHOZN CHO-K1 stable cell line service >3 months
1.Vector Construction;
2.Transfection and; screening of polyclonal cell lines;
3.Monoclonal screening;
4.Cryopreservation and quality control of the cell lines;
5.Delivering high quality stable clones
Quote

Related Services

To meet your needs to the fullest extent, Gene Universal also provides Oligo Synthesis,Gene Synthesis,Molecular Biology.

Ordering Information

Or download and fill out the order form below and email to order@geneuniversal.com. Our sales representative will contact you within 24 hours.

  • SEND A MESSAGE

CONTACT US

Gene Universal Inc.

Address:200 Continental Dr. Suite 401, office 402, Newark DE 19713

Tel:+1 302-235-9792

E-mail: sales@geneuniversal.com

Copyright © 2020 Gene Universal, Inc.