Compared with Bacterial Protein Expression ,there are several advantages of using baculovirus expression system, such as improved solubility, ability to incorporate post-translational modifications and higher yields for secreted proteins. Using our baculovirus expression system,we can establish the best possible protocol for your custom project and then scale up to get the highest expression of your target protein. With the stable experimental system, high expression level and post-translation modification, baculovirus expression is widely recognized as a powerful tool for expressing large-scale proteins.
|No.||Service type||Working day||Comment|
|1||Gene synthesis and subcloning||10-15||pFastBac1, pFastBacHT….|
Bacmid preparation ,transfection and expression evaluation
|14||PCR evaluation and P1 virus preparation|
|3||P2 virus amplification||6||P2 virus amplification and MOI optimization|
|4||Small scale||8||200ml expression and purification|
|5||Large scale||Enquiry||Shaker flask/ bioreactor|
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