Parameters of common dyes used for primer modification


Dye

Maximum absorption wavelength (nm)

Fluorescence emission maximum wavelength (nm)

Molar extinction coefficient

Colour

FAM

495

520

83,000

Green

TET

521

536

73,000

Orange

JOE

520

548

71,000

Pink

HEX

520

548

71,000

Pink

TAMRA

544

576

90,000

Rose

Cy3

550

570

150,000

Red

ROX

576

601

82,000

Red

TexasRed

583

603

107,000

Red

Cy5

650

670

250,000

Violet


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