Electrophoresis Basic Parameters

Separation range (bp)

Recommended % agarose

Separation range (bp)

Recommended % 
Polyacrylamide Gel

1,000~30,000

0.5%

100~1,000

3.5%

800~12,000

0.7%

80~500

5.0%

500~10,000

1.0%

60~400

8.0%

400~7,000

1.2%

40~200

12.0%

200~4,000

1.5%

5~100

20.0%

50~2,000

2.0%

 

 


PAGE concentration

Size of fragments in nucleotides, bp

Bromophenol blue

Xylene Cyanol FF

3.5%

100

460

5.0%

65

260

8.0%

45

160

12.0%

20

70

15.0%

15

60

20.0%

12

45


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