Vector List

Standard Cloning Vector
Vector Name Resistance Promoter
pUC57 AmpR NA
pUC57-Kan KanR NA
pUC57-BsaI-Free AmpR NA
pUC19 AmpR NA
pUC18 AmpR NA
pUC57-1.8K AmpR NA
pUC57-Simple AmpR NA
pBlueScript II SK(+) AmpR T7/T3

Baculovirus/Insect Expression Vectors
Vector Name Resistance Promoter
pFastBac1 AmpR/GmR polyhedrin
pFastBac-Dual AmpR/GmR p10 & polyhedrin

Mammalian Expression Vectors
Vector Name Resistance Promoter Special Instructions
pcDNA3.1(+) NeoR/KanR/AmpR CMV /T7
pcDNA3.1(-) NeoR/KanR/AmpR CMV /T7
pcDNA3.1(+) myc-His A NeoR/KanR/AmpR CMV /T7 C- Myc tag and 6xHis tag
pcDNA3.1(+) myc-His B NeoR/KanR/AmpR CMV /T7 C- Myc tag and 6xHis tag
pcDNA3.1(+) myc-His C NeoR/KanR/AmpR CMV /T7 C- Myc tag and 6xHis tag
pcDNA3.1 myc-His(-) A NeoR/KanR/AmpR CMV /T7 C- Myc tag and 6xHis tag
pcDNA3.1 myc-His(-) C NeoR/KanR/AmpR CMV /T7 C- Myc tag and 6xHis tag
pcDNA3.1(+)-EGFP NeoR/KanR/AmpR CMV /T7 C- EGFP tag
pcDNA3.1/Hygro(+) HygR/AmpR CMV /T7
pcDNA3.1/Hygro(-) HygR/AmpR CMV /T7
pcDNA3.1/Zeo (+) BleoR/AmpR CMV /T7
pcDNA3.1/Zeo (-) BleoR/AmpR CMV /T7
psiCHECK2 AmpR HSV TK /T7
pEGFP-N1 NeoR/KanR CMV C- EGFP tag
pEGFP-C1 NeoR/KanR CMV N- EGFP tag
pGL3-basic AmpR NA C- luciferase tag
pGL3-Promoter AmpR SV40 C- luciferase tag

Yeast Expression Vectors
Vector Name Resistance Promoter Special Instructions
pPIC 3.5k AmpR AOX1
pPIC9 AmpR AOX1 N- α-factor secretion signal
pPIC9K AmpR/KanR AOX1 N- α-factor secretion signal
pPICZA Zeocin AOX1 C- Myc and 6*His
pPICZB Zeocin AOX1 C- Myc and 6*His
pPICZC Zeocin AOX1 C- Myc and 6*His
pPICZαA Zeocin AOX1 N- α-factor secretion signal C- Myc and 6*His
pPICZαB Zeocin AOX1 N- α-factor secretion signal C- Myc and 6*His
pPICZαC Zeocin AOX1 N- α-factor secretion signal C- Myc and 6*His

Bacterial Expression Vectors
Vector Name Resistance Promoter Tags(N-terminal) Tags(C-terminal) Protease Cleavage Sites
pET-3a AmpR

T7

N- T7.Tag

pET-3d AmpR T7 N- T7.Tag

pET-9a KanR

T7

N- T7.Tag

pET-11a AmpR T7 N- T7.Tag

pET-11b AmpR

T7

N- T7.Tag

pET-11d AmpR T7 N- T7.Tag

pET-15b AmpR

T7

N- His.Tag
Thrombin site
pET-16b AmpR T7 N- His.Tag
Factor Xa site
pET-17b AmpR

T7

N- T7.Tag

pET-19b AmpR T7 N- His.Tag
Enterokinase site
pET-20b(+) AmpR

T7

N- His.Tag C- Pelb signal
pET-21a(+) AmpR T7 N- T7.Tag C- His.Tag
pET-21b(+) AmpR

T7

N- T7.Tag C- His.Tag
pET-21d(+) AmpR T7 N- T7.Tag C- His.Tag
pET-22b(+) AmpR

T7

N- Pelb signal C- His.Tag
pET-23a(+) AmpR T7 N- T7.Tag C- His.Tag
pET-24a(+) KanR

T7

N- T7.Tag C- His.Tag
pET-24b(+) KanR T7 N- T7.Tag C- His.Tag
pET-24c(+) KanR

T7

N- T7.Tag C- His.Tag
pET-24d(+) KanR T7 N- T7.Tag C- His.Tag
pET-25b(+) AmpR

T7

N- Pelb signal C- Hsv.Tag and His.Tag
pET-26b(+) KanR T7 N- Pelb signal C- His.Tag
pET-27b(+) KanR

T7

N- Pelb signal C- Hsv.Tag and His.Tag
pET-28a(+) KanR T7 N- His.Tag and T7.Tag C- His.Tag Thrombin site
pET-28b(+) KanR

T7

N- His.Tag and T7.Tag C- His.Tag Thrombin site
pET-29a(+) KanR T7 N- S.Tag C- His.Tag Thrombin site
pET-29b(+) KanR

T7

N- S.Tag C- His.Tag Thrombin site
pET-30a(+) KanR T7 N- His.Tag and S.Tag C- His.Tag Thrombin site and Enterokinase site
pET-30b(+) KanR

T7

N- His.Tag and S.Tag C- His.Tag Thrombin site and Enterokinase site
pET-31b(+) AmpR T7 N- KSI and His.Tag C- His.Tag
pET-32a(+) AmpR

T7

N- TrxA and His.Tag and S.Tag C- His.Tag Thrombin site and Enterokinase site
pET-32b(+) AmpR T7 N- TrxA and His.Tag and S.Tag C- His.Tag Thrombin site and Enterokinase site
pET-41a(+) KanR

T7

N- GST.Tag and His.Tag and S.Tag C- 8*His.Tag Thrombin site and Enterokinase site
pET-41b(+) KanR T7 N- GST.Tag and His.Tag and S.Tag C- 8*His.Tag Thrombin site and Enterokinase site
pET-42b(+) KanR

T7

N- GST.Tag and His.Tag and S.Tag C- 8*His.Tag Thrombin site and Factor Xa site
pET-43.1a(+) AmpR T7 N- NusA and His.Tag and S.Tag C- Hsv.Tag and His.Tag Thrombin site
pET-45b(+) AmpR

T7

N- His.Tag C- S.Tag Enterokinase site
pET-51b(+) AmpR T7 N- Strep-Tag II C- 10*His.Tag HRV 3C site
pET-52b(+) AmpR

T7

N- Strep-Tag II C- 10*His.Tag HRV 3C site
pGEX-4T-1 AmpR tac N- GST.Tag
Thrombin site
pGEX-4T-2 AmpR

tac

N- GST.Tag
Thrombin site
pGEX-5X-1 AmpR tac N- GST.Tag
Factor Xa site
pGEX-6P-1 AmpR

tac

N- GST.Tag
PreScission site
pGEX-6P-3 AmpR tac N- GST.Tag
PreScission site
pCDFDuet-1 SmR

T7


C- S.Tag
pMAL-c4x AmpR tac N- MBP
Factor Xa site
pMAL-c5E AmpR

tac

N- MBP
Enterokinase site
pMAL-c5x AmpR tac N- MBP
Factor Xa site
pMAL-p5E AmpR

tac

N- MBP
Enterokinase site
pMAl-p5x AmpR tac N- MBP
Factor Xa site
pQE-30 AmpR

T5

N- His.Tag

pQE-60 AmpR T5 N- His.Tag

pGS-21a AmpR T7 N- His.Tag and GST.Tag
Enterokinase site
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