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T4 DNA Ligase and Quick buffer
T4 DNA ligase is often used to catalyze the ligation of double-stranded DNA ends or complementary cohesive ends, and also catalyzes the linkage between the double-stranded RNA 5'- phosphate terminal and the 3'-hydroxyl terminus. It is also possible to repair single strand breaks in double stranded DNA, RNA, or the single strand breaks in DNA/RNA hybrid double strands. All the above reactions are required to consume ATP.
Reaction system
| Component | Volume（μL） |
|---|---|
| 2×Quick Buffer | 5.0 |
| Vector DNA | A |
| Fragment DNA | B |
| Enzyme | 0.5~1.0 |
| ddH2O | Up to 10 μL |
Reaction conditions
(1)Cohesive-ended connection:Reaction at 25℃ for 10~15 min;(2)Blunt-ended connection:Reaction at 25℃ for 30~40 min;
(3)Cohesive-ended connection/Blunt-ended connection:Reaction at 25℃ for 120 min.
Product specification
| Product names | Catalog Number | Specification | Price |
|---|---|---|---|
| T4 DNA Ligase | CL06020 | 20 T | $25 |
| 2×Quick Buffer | $25 | ||
| T4 DNA Ligase | CL06050 | 50 T | $50 |
| 2×Quick Buffer | $50 | ||
| T4 DNA Ligase | CL06100 | 100 T | $90 |
| 2×Quick Buffer | $90 |
Related Services
To meet your needs to the fullest extent, Gene Universal also provides PCR、 Protease Products、 Competent Cell Products.
Ordering Information
Or download and fill out the order form below and email to order@geneuniversal.com. Our sales representative will contact you within 24 hours.
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Fax：+1 302-355-3499
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Tel:+1 302-235-9792
E-mail: sales@geneuniversal.com
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