SUMO Protease(His Tag)

SUMO Protease, also known as Ulp, is a highly active cysteine protease that recognizes the tertiary structure of the SUMO protein, thus the SUMO protein can be cut down from the recombinant fusion protein efficiently and specifically. SUMO Protease maintains a high activity in a wide range of reaction systems such as temperature (4-30 ° C), pH (5.5-9.5), and so on. The SUMO Protease is a recombinant protease that has been purified from E. coli. SUMO Protease has a His-tag which can remove by affinity chromatography.

Storage Condition

It could be preserved for 2 years at -80℃, and preserved for 6 months at -20℃. It is suggested to subpackage into small volume, and avoid repeated freeze-thaw cycles.

Operation Method

1. Made up the follow reaction system in EP tube:
Fusion protein:1mg
SUMO Protease:10U
2. Mixed up and then incubated at suitable temperature, suggest reaction at 4℃ overnight.
3. Analyzed by SDS-PAGE after reaction, and SUMO Protease could be removed by His-tag affinity chromatography.

Product Specifications

Product names Catalog Number Specification Price
SUMO Protease MS01050 50U $16
MS01250 250U $70
MS01500 500U $120

Related Services

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