3C Protease (GST Tag)

The PreScission Protease (HRV 3C Protease) produced by GeneUniversal is a human rhinovirus type 14 3C protease expressed in E. coli with GST tag. It could recognize specifically octapeptide sequence Leu-Glu-Val-Leu-Phe-Gln-Gly-Pro or core pentapeptide sequence Leu-Phe-Gln-Gly-Pro at low temperature (4℃), and digest between glutamine and glycine. It is often used for removing affinity tag, such as Glutathione S-transferase(GST)tag, 6×His tag or other affinity tag.

Storage Condition

It could be preserved for 2 years at -80℃, and preserved for 6 months at -20℃. It is suggested to subpackage into small volume, and avoid repeated freeze-thaw cycles.

Operation Method

1. Made up the follow reaction system in EP tube:
Fusion protein:1mg
Prescission Protease:10U
2. Mixed up and then incubated at suitable temperature, suggest reaction at 4℃ overnight.
3. Analyzed by SDS-PAGE after reaction, and Prescission Protein could be removed by GST tag affinity chromatography.

Product Specifications

Product names Catalog Number Specification Price
PreScission Protease MC01050 50U $16
MC01250 250U $70
MC01500 500U $120

Related Services

To meet your needs to the fullest extent, Gene Universal also provides PCR、 Molecular Cloning and DNA Marker Product、 Competent Cell Products.

Ordering Information

Or download and fill out the order form below and email to order@geneuniversal.com. Our sales representative will contact you within 24 hours.

Copyright © 2024 Gene Universal, Inc.