GUeasy Hot Start Taq DNA polymerase

Product Description

This product is a hot-start Taq enzyme obtained by mixing GUeasy Plus Taq Antibody and GUeasy Taq DNA polymerase in an optimal ratio. After the enzyme is heat-activated at 95°C, the antibody falls off to release the enzyme activity. Before the amplification system is prepared for PCR amplification, non-specific amplification due to primer mispairing will not occur, the non-specific amplification and the formation of primer dimers will be minimized, and the sensitivity and specificity of amplification will be improved.

Advantages

  • Stability:High standard production process achieves extremely high stability.
  • Sensitive:It can effectively detect low copy number templates.
  • Specific:Effectively inhibits primer-dimers.
  • Compatibility:It has excellent compatibility with various PCR and qPCR systems.

Reaction System

Components Volume(ul) Final Conc.
5×GUeasy Hot Start Taq buffer(Mg2+ 4
GUeasy Hot Start Taq DNA polymerase, 5U/ul 1 0.2U/ul
Probe Mix(10uM) 0.5 each 0.2uM
Primer Mix(10uM) 1 each 0.4uM
Template 1-10ul -
ddH2O up to 20ul -

Case Study

Calf thymus genome DNA Template qPCR:Amplification efficiency 97.3%

Product Specification

Product Catalog # Size Activity Price
GUeasy Hot Start Taq DNA polymerase KQ3001-02 200ul 1000U Inquiry
KQ3001-05 500ul 2500U Inquiry
KQ3001-1 1ml 5000U Inquiry
KQ3001-3 3ml 15000U Inquiry
KQ3001-5 5ml 25000U Inquiry

* More discount is available for bulk order


Storage condition

Shipped in ice-pack, stored at -20°C, valid for two years.

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Ordering Information

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