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GUeasy MMLV Reverse-Transcriptase III（H-）
Product Description
M-MLV Reverse Transcriptase (MMLV) is an RNA-mediated DNA polymerase derived from Moloney Murine Leukemia Virus. This enzyme uses RNA (synthesized from cDNA) or ssDNA as a template to make a complementary strand of DNA. Compared with wild-type M-MLV reverse transcriptase, GUeasy MMLV Reverse-Transcriptase III（H-）can effectively synthesize high-quality cDNA by removing RNase H activity through genetic modification and increasing the reverse transcription temperature to 50-60 ℃, avoiding the inhibition of cDNA synthesis by the complex secondary structure of RNA.
Advantages
- Stability:extremely high stability by high-standard production process.
- Tolerance:for common impurities have a strong tolerance.
- Compatibility:compatible with animal, plant, virus and other templates.
Operation steps
| Components | Volume（ul） |
|---|---|
| The previous reaction liquid | 10 |
| 5×GUeasy MMLV Reverse-Transcriptase III buffer | 4 |
| dNTP Mix (10 mM) | 1 |
| GUeasy MMLV Reverse-Transcriptase III（200U/ul） | 1 |
| RNase inhibitor (40 U/µL) | 1 |
| RNase Free ddH2O | up to 20ul |
Quality Control：
| SDS-PAGE purity >98% | No exonuclease activity |
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| No endonuclease activity | Low E.coli genome carryover |
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Product Specification
| Product | Catalog # | Size | Activity | Price |
|---|---|---|---|---|
| GUeasy MMLV Reverse-Transcriptase III（H-） | RT3003-01 | 100ul | 20000U | Inquiry |
| RT3003-02 | 200ul | 40000U | Inquiry | |
| RT3003-05 | 500ul | 100000U | Inquiry | |
| RT3003-1 | 1ml | 200000U | Inquiry | |
| RT3003-3 | 3ml | 600000U | Inquiry | |
| RT3003-5 | 5ml | 1000000U | Inquiry |
* More discount is available for bulk order
Notes
- 1）When using Random Primers, incubate for 5 min at 25℃.This step may be omitted if Oligo (dT)18 or Gene Specific Primers are used.
- 2）Reverse transcription temperature: 42℃ is recommended. For templates with high GC content or complex secondary structure, the reverse transcription temperature can be increased to 50-55℃.
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3）Heating at 85℃ for 5 min was used to inactivate reverse transcriptase.
※ Reverse transcription products can be immediately used for subsequent PCR or qPCR reaction, or can be stored at -20℃ for a short time. If long-term storage is needed, it is recommended to store them at -80℃ after packaging to avoid repeated freeze-thaw.
Storage condition
Shipped in ice-pack, stored at -20°C, valid for two years.
Related Services
To meet your needs to the fullest extent, Gene Universal also provides Molecular Cloning and DNA Marker Product、 Protease Products、 Competent Cell Products.
Ordering Information
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