This kit uses genetically modified MMLV Reverse Transcriptase to efficiently synthesize the first-strand cDNA, and uses the Taq DNA Polymerase, which is blocked by antibodies, for quantitative amplification. This kit mainly contains optimized 2×GUeasy RT-qPCR NM buffer mix,GUeasy RT Enzyme mix and RNase Free ddH2O. It needs to be added according to the reaction system. The buffer contains Mg2+ and dNTP, etc., and the factors that can effectively inhibit non-specific PCR amplification and improve the amplification efficiency of multiple qPCR reaction are added to ensure the amplification efficiency and simultaneously carry out multiple reactions.
Components | Volume(ul) | Final Conc |
---|---|---|
2×GUeasy RT-qPCR NM buffer mix | 10 | 1× |
GUeasy RT Enzyme mix | 1 | - |
Probe Mix(10uM) | 0.5 each | 0.2uM |
Primer Mix(10uM) | 1 each | 0.4uM |
Template | 1-5ul | - |
RNase Free ddH2O | up to 20ul | - |
System: Chicken Liver RNA template quadruple RT-qPCR
Tested products: GU company, A company, B company
Equipment:SLAN-96S
FAM channel:GU————;A————;B————: | HEX channel:GU————;A————;B————: |
ROX channel:GU————;A————;B————: | Cy5 channel:GU————;A————;B————: |
Product | Catalog # | Size | Unit | Price |
---|---|---|---|---|
2×GUeasy One Step RT-qPCR Probe Kit | RTM3003-1 | 1mL | 100rxn | Inquiry |
RTM3003-3 | 3ml | 300rxn | Inquiry | |
RTM3003-5 | 5ml | 500rxn | Inquiry | |
RTM3003-10 | 10ml | 1000rxn | Inquiry | |
RTM3003-30 | 30ml | 3000rxn | Inquiry | |
RTM3003-50 | 50ml | 5000rxn | Inquiry |
* More discount is available for bulk order
Shipped in ice-pack, stored at -20°C, valid for two years.
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