2×GUeasy One Step RT-PCR Probe Kit

Product Description

This kit uses genetically modified MMLV Reverse Transcriptase to efficiently synthesize the first-strand cDNA, and uses the Taq DNA Polymerase, which is blocked by antibodies, for quantitative amplification. This kit mainly contains optimized 2×GUeasy RT-qPCR NM buffer mix,GUeasy RT Enzyme mix and RNase Free ddH2O. It needs to be added according to the reaction system. The buffer contains Mg2+ and dNTP, etc., and the factors that can effectively inhibit non-specific PCR amplification and improve the amplification efficiency of multiple qPCR reaction are added to ensure the amplification efficiency and simultaneously carry out multiple reactions.

Advantages

  • Fast: The one-step method can be used to detect or quantify RNA in animals, plants and microorganisms quickly and accurately.
  • Sensitivity: effective for low-copy templates.
  • Repeatability:Optimized reaction system, ensures repeatability between experiments.

Reaction System

Components Volume(ul) Final Conc
2×GUeasy RT-qPCR NM buffer mix 10
GUeasy RT Enzyme mix 1 -
Probe Mix(10uM) 0.5 each 0.2uM
Primer Mix(10uM) 1 each 0.4uM
Template 1-5ul -
RNase Free ddH2O up to 20ul -

Case study

System: Chicken Liver RNA template quadruple RT-qPCR

Tested products: GU company, A company, B company

Equipment:SLAN-96S

FAM channel:GU————;A————;B———— HEX channel:GU————;A————;B————
ROX channel:GU————;A————;B———— Cy5 channel:GU————;A————;B————

Product Specification

Product Catalog # Size Unit Price
2×GUeasy One Step RT-qPCR Probe Kit RTM3003-1 1mL 100rxn Inquiry
RTM3003-3 3ml 300rxn Inquiry
RTM3003-5 5ml 500rxn Inquiry
RTM3003-10 10ml 1000rxn Inquiry
RTM3003-30 30ml 3000rxn Inquiry
RTM3003-50 50ml 5000rxn Inquiry

* More discount is available for bulk order


Notes

  • 1)Be sure to mix well before use, and avoid excessive bubbles caused by violent vibration
  • 2)Primer concentration: Each Primer Mix contains multiple pairs of primers, and usually the final concentration of each primer is 0.25μM, it can also be adjusted between 0.1-0.5 μM according to the application.
  • 3)Probe concentration: Each Probe Mix contains multiple probes with different fluorescence signals, and the concentration of each probe can be adjusted between 50-300 nM according to the application.
  • 4)Template dilution: Due to the high-sensitive of qPCR, diluting the template is recommended. To control CT value is appropriate between 20 and 35.
  • 5)Reaction system: To ensure the effectiveness and repeatability of GOI (gene of interest) amplification, 20μL or 50μL is recommended.
  • 6)Reaction system preparation: To avoid cross-contamination and aerosol-contamination, nuclease-free pipettes (with filter inside) and tubes are recommended, all the reaction system preparation needs to be done in clean bench.
  • 7)Annealing/Extension: Reaction temperature and time can be adjusted according to the designed primer Tm value.

Storage condition

Shipped in ice-pack, stored at -20°C, valid for two years.

Related Services

To meet your needs to the fullest extent, Gene Universal also provides Molecular Cloning and DNA Marker Product、 Protease Products Competent Cell Products.

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