This kit will efficiently synthesize the first-strand cDNA using genetically modified MMLV Reverse Transcriptase, and the Taq DNA Polymerase which is blocked by antibodies, for quantitative amplification. This kit mainly contains optimized 2×GUeasy RT-qPCR NM Buffer mix, GUeasy RT Enzyme mix (U+, RNasin) and RNase Free ddH2O, which are added according to the reaction system. The buffer contains Mg2+ and dNTP, etc., and the factors that can effectively inhibit non-specific PCR amplification and improve the amplification efficiency of multiple qPCR reaction are added to ensure the amplification efficiency and simultaneously carry out multiple reactions. In addition, UDG / dUTP anti-pollution system ensures the authenticity and reliability of the results even in room temperature. Meanwhile, our deeply optimized multiple reaction buffer system, significantly improves the amplification efficiency, promotes the effective amplification of low concentration template, as well as carries out high-sensitivity reaction.
Components | Volume(ul) | Final Conc |
---|---|---|
2×GUeasy RT-qPCR NM buffer mix | 10 | 1× |
GUeasy RT Enzyme mix(U+,RNasin) | 1 | - |
Probe Mix(10uM) | 0.5 each | 0.2uM |
Primer Mix(10uM) | 1 each | 0.4uM |
Template | 1-5ul | - |
RNase Free ddH2O | up to 20ul | - |
System: Chicken Liver RNA template quadruple RT-qPCR
Tested products: GU company, A company, B company
Equipment:SLAN-96S
FAM channel:GU————;A————;B————: | HEX channel:GU————;A————;B————: |
ROX channel:GU————;A————;B————: | Cy5 channel:GU————;A————;B————: |
Product | Catalog # | Size | Unit | Price |
---|---|---|---|---|
2×GUeasy One Step RT-qPCR Probe U+ Kit | RTMU3003-1 | 1mL | 100rxn | Inquiry |
RTMU3003-3 | 3ml | 300rxn | Inquiry | |
RTMU3003-5 | 5ml | 500rxn | Inquiry | |
RTMU3003-10 | 10ml | 1000rxn | Inquiry | |
RTMU3003-30 | 30ml | 3000rxn | Inquiry | |
RTMU3003-50 | 50ml | 5000rxn | Inquiry |
* More discount is available for bulk order
Shipped in ice-pack, stored at -20°C, valid for two years.
To meet your needs to the fullest extent, Gene Universal also provides Molecular Cloning and DNA Marker Product、 Protease Products、 Competent Cell Products.
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