Gene Universal

The expression of exogenous genes in target cells usually requires the introduction of viral or non-viral vectors containing exogenous DNA fragments. In clinical applications, using the traditional methods that direct introduction of exogenous DNA into the human body have certain safety issues. In contrast, in vitro transcribed(IVT) mRNA has natural advantages in safety due to its non infectious ability, unconformity genome and natural degradation in cells. Therefore, it is also a popular tool for gene therapy and new vaccine research.

Advantages

Gene Universal have professional and rich experience technical team for custom IVT mRNA synthesis, now offers one-stop service from Gene synthesis, Plasmid Preparation, linearized plasmid to IVT mRNA synthesis. The length range of IVT mRNA is from 100nt to 5000nt, it can be optimized and customized for different downstream applications, such as template optimization, coding region codon optimization, nucleoside modification, and post transcriptional modification, etc. IVT mRNA can be applied in research fields such as cell transfection, embryo injection, bio-pharmaceuticals, and vaccine development, etc.

Service Features

Free codon optimization, improving mRNA translation efficiency
Optimized vector, have higher IVT efficiency
Custom cap and tail: 5’ cap(Uncapped、Cap0、Cap1) and 3’ PolyA Tail(No Tail、120A Tail, customized), to enhance mRNA stability and translation efficiency
Modifications like 5-Methyl-CTP、Pseudo-UTP, which can enhance the mRNA translation efficiency and immune evasion performance
Comprehensive quality control plan, customized QC projects according to needs
One-stop service from DNA template synthesis to IVT mRNA
Rapid delivery – Industry-leading turnaround time

Service Options

Services Step	Service Content	Turnaround Time	Price
1. Gene synthesis, Plasmid Preparation	Gene synthesis、Plasmid construction	2-3 weeks	Inquiry
1. Gene synthesis, Plasmid Preparation	Plasmid preparation	2-3 weeks
2. IVT mRNA Synthesis	Linearized plasmid	3-4weeks
2. IVT mRNA Synthesis	IVT, add 5'cap and 3'PolyA tail, modification	3-4weeks
3. Quality Control	The default QC+extra QC item(Customized)	Inquiry

QC Item

QC	Item	Test Method	Specification
Default	mRNA Length	Agarose gel electrophoresis	Expected size band detected
	mRNA Sequence	Sanger sequencing	Correct DNA template
	Concentration	Nano drop or Qubit	1μg/μl(Default)
	Appearance	Visual inspect	Clear, no visible particles
	A260/280	Nano drop	2.0-2.2
	Purity	HPLC	≥90%
Extra(optional)*	Residual DNA template	qPCR	≤ 10pg/ug
	Endotoxin level	Gel-TAL	≤0.005 EU/μg
	Residual Protein	ELISA or Qubit	≤ 1%
	Bioburden	Plate	48h sterile spots