Site-directed Mutagenesis

Site-directed Mutagenesis
  • Overview
  • Features
  • Service Descriptions
  • Service Procedure
  • Service Options
  • Standard Deliverables

Site-directed mutagenesis introduces changes to DNA fragments by PCR mutagenesis, including deletion, insertion and point mutation, to enable studies of gene regulation, DNA-protein interactions, protein structure/function, enzyme active sites and novel protein.Your time is valuable,and please spend it on research,just design the mutation projects and submit to Gene Universal,we will deliver you the mutant constructs with 100% accuracy.

Features

● 100% accuracy: Mutant constructs are sequence-verified via Sanger sequencing

● Unlimited Sites: With our sophisticated gene synthesis and mutagenesis technology, Gene Universal can introduce mutations at any site

● Most Competitive Prices starting from $99/mutant

Service Descriptions

We highly recommend you to choose our mutagenesis bundled with gene synthesis service.

What we need for your custom DNA template?

● Ship us your DNA template plasmid as starting material. 4 μg of template DNA as the starting material is recommended. If the starting material is bacterial culture, please send the culture at sufficient amount.

● The complete sequence of the template including the target gene and its vector.

● Mutation specifications.

● Maps and antibiotic resistance of the template and destination vectors.

Service Procedure

  • Materials provided

    by customer

  • QC identification

  • Design and optimize

    the primer

  • Cloned into original

    vector or appointed vector

  • Sequencing & Validation

  • Delivery

Service Options

Length Mutated Site EDT Price
< 3000 bp One 5-8 Business Days $99/mutation
Two 10-14 Business Days $149/mutation
Three 15-20 Business Days $199/mutation
3000 bp-8000 bp One 5-8 Business Days $129/mutation
Two 10-14 Business Days $179/mutation
Three 15-20 Business Days $229/mutation
> 8000 bp One Inquiry Inquire
Two Inquiry Inquire
Three Inquiry Inquire

One mutation is defined as any combination of base changes within a 30-base frame.

Standard Deliverables

- 1tube of 5 μg of lyophilized plasmid containing target gene.

- Sequence chromatograms covering your gene (electronic).

- Construct map for the plasmid (electronic).

- Quality assurance certificate.

REQUEST A QUOTE

QUOTE/ORDER

Download and fill out the order form below and email to order@geneuniversal.com or sumbit your project through our GeneEasy online quote system . Our sales representative will contact you within 24 hours.

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