Site-directed mutagenesis introduces changes to DNA fragments by PCR mutagenesis, including deletion, insertion and point mutation, to enable studies of gene regulation, DNA-protein interactions, protein structure/function, enzyme active sites and novel protein.Your time is valuable,and please spend it on research,just design the mutation projects and submit to Gene Universal,we will deliver you the mutant constructs with 100% accuracy.
Features
● 100% accuracy: Mutant constructs are sequence-verified via Sanger sequencing
● Unlimited Sites: With our sophisticated gene synthesis and mutagenesis technology, Gene Universal can introduce mutations at any site
● Most Competitive Prices starting from $99/mutant
Service Descriptions
We highly recommend you to choose our mutagenesis bundled with gene synthesis service.
What we need for your custom DNA template?
● Ship us your DNA template plasmid as starting material. 4 μg of template DNA as the starting material is recommended. If the starting material is bacterial culture, please send the culture at sufficient amount.
● The complete sequence of the template including the target gene and its vector.
● Mutation specifications.
● Maps and antibiotic resistance of the template and destination vectors.
Service Procedure
Materials provided
by customer
QC identification
Design and optimize
the primer
Cloned into original
vector or appointed vector
Sequencing & Validation
Delivery
Service Options
Length | Mutated Site | EDT | Price |
---|---|---|---|
< 3000 bp | One | 5-8 Business Days | $99/mutation |
Two | 10-14 Business Days | $149/mutation | |
Three | 15-20 Business Days | $199/mutation | |
3000 bp-8000 bp | One | 5-8 Business Days | $129/mutation |
Two | 10-14 Business Days | $179/mutation | |
Three | 15-20 Business Days | $229/mutation | |
> 8000 bp | One | Inquiry | Inquire |
Two | Inquiry | Inquire | |
Three | Inquiry | Inquire |
One mutation is defined as any combination of base changes within a 30-base frame.
Standard Deliverables
- 1tube of 5 μg of lyophilized plasmid containing target gene.
- Sequence chromatograms covering your gene (electronic).
- Construct map for the plasmid (electronic).
- Quality assurance certificate.
Download and fill out the order form below and email to order@geneuniversal.com or sumbit your project through our GeneEasy online quote system . Our sales representative will contact you within 24 hours.
Phone: +1 302-235-9792
Fax:+1 302-355-3499