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Bacterial Expression
Gene Universal specializes in E.coli protein expression service with risk-free guarantee. Our Guaranteed Bacterial Recombinant Protein Expression Service Package consists of codon optimization service, followed by gene synthesis and subcloning, all the way through protein expression and purification. With the risk-free guarantee, no cost will be charged if the project fails. The overall success rate is over 95% in E.coli expression systems. Place your order today to experience unprecedented, high quality services yet competitive pricing Bacterial Protein Expression Services.
Service Procedure
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Gene Synthesis
& Cloning
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Protein expression evaluation
and optimization
-
Protein purification
pilot study
-
Scale-up with required
Quantity and Purity
-
QC & Shipping
Guaranteed Packages
|
Risk-free E.coli Protein
Service Packages |
Description
|
Amount
|
Timeline
|
Purity
|
Pricing
|
|---|---|---|---|---|---|
|
Risk-free Guaranteed Packages
(Tagged:His/MBP/GST) |
‐Gene Synthesis & cloning
‐Expression pilot study ‐Scale-up and purification ‐Tag removal(optional) ‐Guaranteed quantity of purified protein ‐QC:SDS-PAGE and Western Blot(Tagged) |
3mg(Guaranteed)
|
4-6 weeks
(From gene synthesis to purified proteins) |
85%
|
US$1,450
|
|
90%
|
US$1,850
|
||||
|
95%
|
US$2,250
|
||||
|
5mg(Guaranteed)
|
85%
|
US$1,950
|
|||
|
90%
|
US$2,350
|
||||
|
95%
|
US$2,750
|
||||
|
10mg(Guaranteed)
|
85%
|
US$2,450
|
|||
|
90%
|
US$2,850
|
||||
|
95%
|
US$3,250
|
||||
|
Other Quantity
|
Quote
|
||||
|
Risk-free Guaranteed Packages
(Tag-free) |
3mg(Guaranteed)
|
85%
|
US$2,850
|
||
|
90%
|
US$3,850
|
||||
|
5mg(Guaranteed)
|
85%
|
US$3,950
|
|||
|
90%
|
US$4,950
|
||||
|
10mg(Guaranteed)
|
85%
|
US$6,050
|
|||
|
90%
|
US$7,050
|
||||
|
Other Quantity
|
Quote
|
||||
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1. Risk-free for Guaranteed package: Quantity & Purity guaranteed,or it is FREE.
2. Pricing includes gene synthesis <3000 bp.
3. Pricing does not include endotoxin removal or some specific requirements. Additional charges apply for these services.
4. Protein solubility is guaranteed but we DO NOT guarantee the
bioactivity or functionality. If the protein expresses as inclusion bodies, refolding will be attempted to make the protein soluble.5. Protein is delivered in lyophilized powder whenever possible. If the solubility of protein changes after lyophilization, it will be shipped in liquid form.
-
6. For tagged packages, we may use affinity tags to purify a protein and the final protein would be tag containing. For tag-free packages, we can provide service start with tag-free protein expression, and purified by SEC and ion exchange to ensure proper native folding, or start with fusion tag expression, and purified by tag removal, HIC, SEC or IEX to ensure proper native folding.
7. Protein purity is determined by SDS-PAGE, and quantity is determined by Bradford/BCA/A280 assay.Purity determined by HPLC is also available, please inquire for specific requirements.
Custom Package (Charged by steps)
|
Steps
|
Specification
|
Timeline
|
Price
|
Deliverables
|
|---|---|---|---|---|
|
1.Gene synthesis or Subcloning,
Plasmid Prep |
A).Gene synthesis sub cloning into expression vector
|
Varies
(Please inquire) |
$0.15/bp (min:$79/gene)
|
‐Weekly progress reports
‐Desired quantity of purified, ‐Plasmid(synthesized by us,2-5ug) ‐QC data |
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B).Or provide your expression constructs, we’ll sub-clone your gene into our expression vector
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$99/construct
|
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2.Expression Pilot Study
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Protein expression evaluation and optimization
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$199
|
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3.Protein Purification
|
Optimize and find the purification conditions of your protein (purity>85%)
|
$699
|
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4.Scale-up and purification
|
Large scale expression & purification at your desired quantity (mg to g)
|
Quote
|
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5.Tag removal and endotoxin removal and other steps(Optional)
|
Perform tag removal ,endotoxin removal,higher purity and other steps as needed per your request
|
Quote
|
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6.QC testing
|
SDS-PAGE and Western Blot(tagged protein only)
|
Case Study
Case #1: Interleukin
Challenges
- ●Poor Solubility
- ●Tag-free, Affinity Purification is not Applicable
- ●Easy Polymerization
- ●Intrachain Disulfide Bond
Process Development
- ●Strain screening to promote disulfide bond formation in the cytoplasm
- ●Medium optimization, feeding strategy, especially harvest timing
- ●IEX Capture
- ●SEC Purification
- ●Buffer Exchange
QC Data:
-
M:Protein Marker
R: Reducing
NR:Non-Reducing
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Yield: 350 mg/L, SDS-PAGE > 95%, SEC-HPLC > 95%
Case #2: Somatotropin
Challenges
- ●Inclusion Body Expression& Purification
- ●Disulfide Bond
- ●Easy Polymerization
- ●Unfolding & Refolding
Process Development
- ●Medium optimization, fermentation process control
- ●Dilution Refodling: dilution method/factors/speed
- ●Screening denaturants, stabilizers, aggregation inhibitors, pH, oxidant, reductant
- ●HIC purificatin, salt gradient elution
QC Data:
-
M:Protein Marker
R: Reducing
NR:Non-Reducing
-
Yield: 500 mg/L, SDS-PAGE > 95%, SEC-HPLC > 95%
REQUEST A QUOTE
Download and fill out the order form below and email to order@geneuniversal.com or sumbit your project through our GeneEasy online quote system . Our sales representative will contact you within 24 hours.
Phone: +1 302-235-9792
Fax：+1 302-355-3499
Other Services
More recombinant protein production platforms:
CONTACT US
Gene Universal Inc.
Address: 200 Continental Dr. Suite 401, office 402, Newark DE 19713
Tel:+1 302-235-9792
E-mail: sales@geneuniversal.com
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