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Insect Cell Expression
Compared with Bacterial Protein Expression ,there are several advantages of using baculovirus expression system, such as improved solubility, ability to incorporate post-translational modifications and higher yields for secreted proteins. Using our baculovirus expression system,we can establish the best possible protocol for your custom project and then scale up to get the highest expression of your target protein. With the stable experimental system, high expression level and post-translation modification, baculovirus expression is widely recognized as a powerful tool for expressing large-scale proteins.
Service Descriptions
● Our baculovirus expression Services start with Gene Universals’codon optimization service, followed by gene synthesis and sub cloning, all the way through protein expression and purification using our bacterial protein expression system.
● Gene Universal possesses the most advanced AKTA purifier system, along with the pre-positioned Ion column, molecular sieve, hydrophobic interaction column and affinity column.
● Gene Universal’s Endotoxin Removal Service can effectively reduce the endotoxin level to under 10 EU/mg during recombinant protein production with a success rate of 98%.
Service Procedure
Service Options
Bac-to-Bac system service type
| No. | Service type | Working day | Comment |
|---|---|---|---|
| 1 | Gene synthesis and subcloning | 10-15 | pFastBac1, pFastBacHT…. |
| 2 |
Bacmid preparation ,transfection and expression evaluation |
14 | PCR evaluation and P1 virus preparation |
| 3 | P2 virus amplification | 6 | P2 virus amplification and MOI optimization |
| 4 | Small scale | 8 | 200ml expression and purification |
| 5 | Large scale | Enquiry | Shaker flask/ bioreactor |
Terms and Conditions
1. We will start the project according to your choice and will only charge the cost step by step.
2. Affinity purification, ion exchange purification, hydrophobic purification and SEC purification can be used. The expression system will affect the final protein purity and activity.
REQUEST A QUOTE
Download and fill out the order form below and email to order@geneuniversal.com or sumbit your project through our GeneEasy online quote system . Our sales representative will contact you within 24 hours.
Phone: +1 302-235-9792
Fax：+1 302-355-3499
Other Services
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CONTACT US
Gene Universal Inc.
Address: 200 Continental Dr. Suite 401, office 402, Newark DE 19713
Tel:+1 302-235-9792
E-mail: sales@geneuniversal.com
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