The turnaround time for gene synthesis generally ranges from 5 business days. This timeframe is dependent on factors such as the gene length, the complexity of the sequence, and any specific modifications that may be requested. For more complex genes or those requiring additional steps like cloning or vector construction, the turnaround time may extend slightly. We aim to balance speed with precision to ensure high-quality results.

We are capable of synthesizing genes of various lengths, ranging from short oligonucleotides to full-length genes exceeding 20,000 base pairs. The synthesis process is carefully tailored to the gene length, with longer or more complex genes requiring additional time and resources to ensure accuracy and efficiency. Our advanced technology and expertise allow us to handle a wide range of gene sizes, making us a versatile partner for your gene synthesis needs.

Yes, we offer comprehensive codon optimization services, which are tailored to the expression system of your choice. Whether you are expressing the gene in bacterial, yeast, insect, or mammalian cells, we can optimize the codon usage to enhance expression levels. This service includes a detailed analysis of the codon usage bias in the target organism, ensuring that the synthesized gene is designed for maximum efficiency and protein yield in the chosen system.

Absolutely, we specialize in synthesizing genes with complex sequences, including those with high GC content, repetitive elements, or secondary structures that can make synthesis challenging. Our advanced methodologies and quality control processes allow us to successfully produce accurate sequences even under these challenging conditions. We take extra care in designing and optimizing the synthesis process to overcome these difficulties, ensuring reliable and high-quality gene synthesis.

We ensure the accuracy of each synthesized gene through a rigorous quality control process. This includes multiple stages of sequence verification, primarily by Sanger sequencing, to confirm that the final product matches the requested sequence with 100% accuracy. Additionally, we may use other methods such as restriction enzyme analysis for complex or long genes. This multi-layered approach guarantees that our synthesized genes meet the highest standards of precision and quality.

Yes, we offer cloning services where the synthesized gene can be inserted into the vector of your choice. Whether you need the gene cloned into a standard vector or a custom one, we can accommodate your requirements. Our cloning services include sequence verification, restriction enzyme analysis, and the option for endotoxin-free plasmid preparation, ensuring that the cloned gene is ready for downstream applications such as expression or functional studies.

We offer a variety of modifications to synthesized genes, including the addition of tags (e.g., His-tag, FLAG-tag), point mutations, deletions, or insertions. Additionally, we can perform gene fusion to create chimeric constructs or add regulatory elements such as promoters, enhancers, or polyadenylation (polyA) signals. These modifications are designed to meet your specific experimental needs and can be customized to optimize gene function in your desired expression system.

We provide recombinant protein expression services using a variety of expression systems, including E. coli, yeast, insect, and mammalian cells. Each system has its own advantages and is selected based on the specific needs of the protein, such as the desired yield, post-translational modifications, and solubility. Our team of experts will work with you to determine the most suitable expression system for your protein of interest, ensuring optimal results for your research or production needs.

The typical yield for recombinant protein production can vary widely depending on several factors, including the nature of the protein, the expression system used, and the scale of production. We typically achieve yields ranging from milligrams to grams, depending on your project's specific requirements. For high-yield projects, especially those using optimized E. coli or mammalian systems, we can often achieve gram-scale production, ensuring ample quantities of high-quality protein for downstream applications.

Yes, we are fully equipped to produce recombinant proteins with various post-translational modifications. These modifications can include glycosylation, phosphorylation, and the formation of disulfide bonds, among others. Such modifications are crucial for the biological activity and stability of many proteins, and they can be efficiently achieved using our insect or mammalian expression systems. Our team carefully monitors the expression process to ensure that the necessary modifications are correctly introduced and maintained.

Yes, we offer a comprehensive range of purification services for recombinant proteins. Our purification techniques include affinity chromatography, ion-exchange chromatography, and size-exclusion chromatography, among others. These methods are selected and optimized based on the specific properties of your protein, such as its size, charge, and solubility. We strive to deliver highly pure recombinant proteins, ensuring that they meet the stringent requirements for research, diagnostic, or therapeutic applications.

We have extensive experience in managing challenging proteins that are difficult to express or purify. To overcome these challenges, we optimize the expression conditions, such as temperature, induction, and media composition, to improve yield and solubility. Additionally, we may employ solubility tags, fusion partners, or specialized refolding protocols to assist in the production and purification of these proteins. Our team is committed to finding innovative solutions to ensure the successful expression and purification of even the most challenging targets.

We can achieve high levels of purity for recombinant proteins, typically exceeding 90% purity, and often reaching 95% or higher, depending on the protein and purification strategy. Our purification process is tailored to the specific properties of the protein, utilizing a combination of techniques such as affinity chromatography, ion-exchange chromatography, and size-exclusion chromatography to remove contaminants and ensure that the final product is of the highest quality, suitable for research or therapeutic applications.

Yes, we provide comprehensive protein characterization services alongside production. This includes techniques such as SDS-PAGE, Western blotting, mass spectrometry, and analytical HPLC to verify the protein's molecular weight, purity, and identity. Additionally, we can perform functional assays to confirm the biological activity of the protein, ensuring that it meets the required specifications for your research or application. Our characterization services help provide a complete understanding of the protein's properties and functionality.

We are capable of producing a wide range of recombinant antibodies, including full-length IgG antibodies, Fab fragments, single-chain variable fragments (scFv), and even more complex formats like bi-specific antibodies. Our recombinant antibody production services are highly customizable, allowing us to tailor the antibody's characteristics to meet the specific needs of your project. Whether you require antibodies for research, diagnostic, or therapeutic purposes, we have the expertise to deliver high-quality products that meet your specifications.

Yes, we offer recombinant antibody production in various species formats, including human, mouse, rabbit, and others. This allows us to tailor the antibody to your specific research or therapeutic needs, whether you're looking for an antibody with a particular species origin for compatibility with certain assays or for preclinical studies. We can also engineer chimeric or fully humanized antibodies based on the original species format.

The typical timeline for recombinant antibody production start from 1 week, depending on the complexity and specific requirements of your project. This timeline includes the antibody gene synthesis, plasmid preparation, transfection, antibody expression, and purification. While the process is thorough to ensure high quality, we also work to optimize efficiency and can offer expedited services for urgent projects. Our goal is to deliver high-quality recombinant antibodies within a timeframe that meets your project deadlines.

Yes, we provide antibody humanization services, which are particularly valuable for therapeutic applications. Our humanization process typically involves CDR grafting, where the complementarity-determining regions (CDRs) of a non-human antibody are transferred onto a human antibody framework. We also offer framework optimization to further reduce immunogenicity and enhance the antibody's compatibility with human immune systems. Our humanization services are designed to retain the antibody's binding specificity and affinity while making it more suitable for use in clinical settings.

To ensure the quality and functionality of our recombinant antibodies, we implement a rigorous series of quality control tests. These include SDS-PAGE for purity analysis, ELISA for antigen-binding specificity, and binding affinity assays such as surface plasmon resonance (SPR) to confirm the antibody's performance. This comprehensive approach ensures that the recombinant antibodies we deliver are not only pure but also highly effective in their intended use.

We provide recombinant antibody production at various scales, ranging from small-scale milligram quantities for research and preclinical studies to large-scale gram quantities for more extensive applications such as diagnostics or therapeutic development. Our production facilities are equipped to handle scale-up processes efficiently, ensuring consistency and quality at every stage of production. We work closely with you to determine the appropriate scale based on your project requirements and future needs.