CRISPR Gene-Edited Cell Lines

OVERVIEW

Gene Universal offers CRISPR gene-edited cell line generation services based on a well-established gRNA/Cas9 ribonucleoprotein (RNP)–based CRISPR genome editing system. We provide customized cell line construction services for gene knockout, gene knock-in, and precise point mutation. The RNP-based CRISPR editing platform features high editing efficiency and reduced off-target effects.

SERVICE HIGHLIGHTS

Expert Support

Our experienced CRISPR gene editing team provides customized cell line generation services tailored to specific research needs, including gene knockout, gene knock-in, and precise point mutations.
Non-Viral Approach

Compared with traditional viral-based methods, our non-viral CRISPR editing strategy offers higher editing efficiency and reduced off-target effects.
Rapid Turnaround

Gene Universal delivers a one-stop solution encompassing sgRNA synthesis, ssDNA synthesis, vector construction, and cell engineering, enabling fast and efficient project delivery.

SERVICE PROCEDURES

Vector Construction

· sgRNA or ssDNA synthesis

· Plasmid construction

Gene Delivery

· Electroporation of RNP complexes

· Electroporation of RNP combined with ssDNA or plasmid

Cell Line establishment

· Antibiotic selection

· Monoclonal cell line establishment

Genotype confirmation and QC

· PCR or qPCR

· Sanger sequencing

· WB Western blot (WB)

· Sterility and mycoplasma testing

SERVICE DETAILS

Service Type	Service Name	Deliverables	Turnaround time(BD)	Price(USD)	Remarks
Knockout	Frameshift Mutation Knockout Cell Line (Sanger sequencing validation)	Target and Wild-type cells, >10^6 cells/tube *2 tubes for each type of cell	60	2850	RNP construction method
Knockout	Frameshift Mutation Knockout Cell Line (Sanger sequencing and Western blot Validation)	Target and Wild-type cells, >10^6 cells/tube *2 tubes for each type of cell	70	3150	RNP construction method
Knockout	Fragment Knockout Cell Line heterozygote（Sanger sequencing validation)	Target and Wild-type cells, >10^6 cells/tube *2 tubes for each type of cell	60	4650	RNP construction method
Knockout	Fragment Knockout Cell Line homozygote（Sanger sequencing validation)	Target and Wild-type cells, >10^6 cells/tube *2 tubes for each type of cell	70	6900	RNP construction method
Knockin	Point Mutation Knockin cell line heterozygote (Sanger sequencing validation)	Target and Wild-type cells, >10^6 cells/tube *2 tubes for each type of cell	70	5700	RNP construction method
Knockin	Point Mutation Knockin cell line homozygote (Sanger sequencing validation)	Target and Wild-type cells, >10^6 cells/tube *2 tubes for each type of cell	70	8100	RNP construction method
Knockin	Fragment Knockin cell line heterozygote (Sanger sequencing validation)	Target and Wild-type cells, >10^6 cells/tube *2 tubes for each type of cell	80	6450	RNP construction method
Knockin	Fragment Knockin cell line heterozygote(Sanger sequencing and Western blot Validation)	Target and Wild-type cells, >10^6 cells/tube *2 tubes for each type of cell	90	6750	RNP construction method
Knockin	Fragment Knockin cell line homozygote (Sanger sequencing validation)	Target and Wild-type cells, >10^6 cells/tube *2 tubes for each type of cell	90	8700	RNP construction method
Knockin	Fragment Knockin cell line homozygote(Sanger sequencing and Western blot Validation)	Target and Wild-type cells, >10^6 cells/tube *2 tubes for each type of cell	100	9000	RNP construction method

Note: Cells, relevant detection antibodies, and any special culture media or special FBS must be provided by the customer.

CASE STUDY

The contributions indicate the inferred sequences present in the edited population and their relative proportions, in contrast to the indel distribution plot, which does not assign specific sequence contributions. Cut sites are shown as black vertical dotted lines, and the wild-type sequence is indicated by a “+” symbol on the far left.

Sanger sequencing comparison of wild-type and edited samples around the guide RNA target site. The black underline indicates the guide sequence, the red underline marks the PAM site, and the dotted line denotes the expected cut site. Mixed base calls downstream of the cut site reflect error-prone repair following genome editing.

44KD Target

37KD GAPDH

WT clone1 clone2 clone3

Figure: Validation of target gene knockout in T24 cells by Sanger sequencing and Western blot analysis.

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