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Affinity Maturation Service

Affinity Maturation Service

OVERVIEW

Affinity maturation refers to the process of enhancing antibody affinity through in vitro experiments when the initial affinity does not meet the requirements for downstream development. Conventional methods involve constructing saturation mutagenesis libraries and high-throughput screening based on mammalian cell expression. We guarantee a more than fivefold improvement in the delivered antibody’s affinity!

SERVICE ADVANTAGES

  • 01AI-assisted antibody back-mutation design significantly improves efficiency and saves time.
  • 03Guaranteed affinity improvement—enhanced by several folds, reaching nanomolar levels or higher.
  • 02Unbiased CDR saturation mutagenesis combined with precise affinity data.

SERVICE PROCEDURE

  • Start >
  • Week 2 >
  • Week 4 >
  • Week 8 >
  • Week 9 >

  • Homology modeling and sequence analysis

    Computer-aided modeling / Key residue analysis

  • Trimer combination library / Single-point saturation mutagenesis library

    Unbiased saturation mutagenesis / Library construction

  • Single-point mutation saturation library high-throughput screening

    96-well plate high-throughput transient expression / BLI high-throughput affinity ranking / Key residue identification

  • Combination mutation screening

    3-20 mL transient expression / BLI affinity ranking / Biacore affinity validation

  • Antibody delivery and Biacore affinity validation

    SDS-PAGE / SEC-HPLC / Biacore affinity confirmation

SERVICE CONTENT

No. Items Service Details EDT Deliverable
1 Sequence analysis and design

※ Unbiased CDR saturation mutagenesis based on client requirements

1 Week

※ 2–3 affinity-matured antibody sequences(at least one with >5-fold affinity improvement)

※ Cloned plasmids

※ Affinity maturation report

※ 2–3 antibody products(1 mg each)

※ COA file

2 Single-point saturation mutation library display

※ Single-point saturation mutation library construction

※ High-throughput mammalian cell secretory expression

3-4 Weeks
3 Affinity testing

※ Culture supernatant ELISA/BLI affinity ranking

※ Key mutation site identification

4 Combination mutation screening

※ Combination mutation design

※ Plasmid construction, transient expression and purification

3-4 Weeks

※ BLI affinity ranking

※ Biacore affinity validation

CASE STUDY

Loading koff(1/s) kon(1/Ms) KD(M) Response Loading Height FullR2
Parent Ab 1.68E-003 1.14E+006 1.47E-009 0.419 0.7652 0.994
Ab mut1 1.56E-04 9.29E+05 1.68E-10 0.4941 0.9019 0.995
Ab mut4 1.38E-04 9.00E+05 1.54E-10 0.4793 0.8818 0.996

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