
OVERVIEW
Antibody humanization involves modifying mouse-derived antibodies through gene cloning and DNA recombination, replacing most of their amino acid sequences with human counterparts. This process retains the affinity and specificity of the original mouse monoclonal antibody while significantly reducing immunogenicity. Humanized antibodies are less likely to trigger immune responses in humans and are crucial for the development of therapeutic antibodies.
Gene Universal offers reliable antibody humanization services based on CDR grafting or surface resurfacing strategies. With an efficient workflow in place, we can complete humanization in as little as 3 weeks.The humanized antibodies retain the full affinity and specificity of their parental counterparts.
Post-translational modifications (PTMs) refer to chemical changes that occur to a protein after translation. During antibody production, storage, and clinical application, various PTM variants may arise. These modifications can alter the antibody’s charge and structure, potentially impacting its affinity for antigens and Fc receptors, and ultimately affecting critical quality attributes such as therapeutic activity. At Gene Universal, we use computational modeling combined with site-saturation mutagenesis libraries to predict and screen for mutants that retain affinity and specificity comparable to the original antibody—helping to streamline downstream therapeutic antibody development.
SERVICE ADVANTAGES
- 01Antibody engineering guided by bioinformatics and structural biology through computer-aided design
- 03Maintains affinity comparable to the original antibody
- 02Simultaneous optimization of both heavy and light chains, achieving >90% humanization
- 04Humanized antibody delivery in as fast as 3 weeks
SERVICE PROCEDURE
- Start >
- Week 1 >
- Week 2 >
- Week 3 >
- Week 4 >
- Week 5 >
-
Sequence Analysis & Homology Modeling
Computational modeling / Germline selection
-
Sequence Design & Vector Construction
Back-mutation / PTM consideration / Site-directed mutation library
-
Transient Expression & Purification
96-well high-throughput plate screening / Small-scale expression(3–30 mL)and purification
-
Affinity Assessment
BLI-based high-throughput affinity ranking / Biacore affinity validation
-
Antibody Delivery & Biacore Validation
SDS-PAGE / SEC-HPLC / Biacore confirmation of affinity
SERVICE CONTENT
Items | Service Details | EDT | Deliverable |
---|---|---|---|
Sequence Analysis |
※ Construct 3D antibody structure models ※ Germline Selction ※ CDR grafting with key residue back mutations ※ Removal of PTM sites (optional) |
1 Week |
※ 2–3 humanized antibody variants(at least one with affinity comparable to the original antibody) ※ Cloned plasmids ※ Humanization design report ※ 2–3 antibody samples(1 mg each) ※ COA |
Antibody Expression |
※ Gene synthesis & vector construction ※ High-throughput expression in mammalian system or small-scale antibody expression |
2 Week | |
Affinity Measurement |
※ ELISA/BLI-based affinity screening from culture supernatant ※ Biacore validation |
1 Week | |
Antibody Expression |
※ Small-scale antibody expression and purification ※ QC |
1 Week |
CASE STUDY


Name | Capture Level(RU) | Analyte | ka(1/Ms) | kd(1/s) | KD(M) | Rmax(RU) | Chi²(RU²) | U-value |
---|---|---|---|---|---|---|---|---|
Parent Ab | 102 | XXX | 8.06E+05 | 1.36E-04 | 1.68E-10 | 60.51 | 0.0288 | 2 |
Humanized Ab | 156 | XXX | 4.28E+05 | 6.04E-05 | 1.41E-10 | 78.85 | 0.035 | 5 |
Request A Quote
Download and fill out the order form below Order form
Please send e-mail to us for quotation sales@geneuniversal.com
Online Request Submission
Have a question about sales or support? Our team is ready to help!