GUeasyCHO-K1 Stable Cell Line Development Service

OVERVIEW

Gene Universal offers high-expression, industry-ready stable cell lines to accelerate your biologics development. Our proprietary GUeasyCHO-K1 cells—sourced from ATCC and optimized for protein and antibody expression—deliver enhanced yield and stability. Backed by extensive production experience, we provide end-to-end solutions from gene to stable cell line. Custom options include FUT8 knockout CHO-K1 and host cell line development based on your needs.

SERVICE ADVANTAGES

01Tailored experimental plans and project feasibility reports based on client needs
03Our GUeasyCHO-K1 system, with in-house vectors, enables rapid optimization for stable expression above 5 g/L.
02Mature and professional stable cell line development platform offers flexible, customized services
04Our stable cell lines ensure stability for 20+ passages with various selection markers

SERVICE PROCEDURE

Gene synthesis & expression vector construction
Stable cell line transfection & stable pool construction
High-expression
clone screening
Identification of stable cell line clones
Establishment of PCB cell bank
PCB cell bank
stability testing

SERVICE CONTENT

GUeasyCHO-K1 Project Development

Steps	Service Content	Turnaround	Deliverable
Sequence design and plasmid construction	Codon optimization and plasmid construction	1~2 Weeks	Plasmid COA report
Cell pool construction	Electroporation optimization	6-9 Weeks	1). Experimental report 2). Top 3 cell lines 3. 60 mL supernatant or protein/antibody after affinity purification 4). Protein expression quality data
Cell pool construction	Cell pool screening	6-9 Weeks
Single clone screening	Cell pool single clone plating	6-9 Weeks
Single clone screening	Single clone screening	6-9 Weeks
Cell bank construction	Cell bank establishment	12 Weeks	1). 20 stable cell lines 2). Complete cell bank records 3). Mycoplasma testing report 4). Target gene sequencing report 5). Cell stability passage data
Cell bank construction	Stability testing	12 Weeks

Customized cell line process development

Steps	Service Content	Turnaround	Deliverable
Customer-specified cell line	Revive and assess cell growth	4 Weeks	1). Optimized culture medium 2). More stable and higher expression culture process
Medium screening to enhance expression levels
Process development to optimize product quality	3 Weeks

Steps

Service Content

Turnaround

Deliverable

Customer-specified cell line

Revive and assess cell growth

4 Weeks

1). Optimized culture medium

2). More stable and higher expression culture process

Medium screening to enhance expression levels

Process development to optimize product quality

3 Weeks