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- Mouse Hybridoma Monoclonal Antibody
OVERVIEW
Mouse monoclonal antibodies are immunoglobulins produced by hybridoma cells that target a single epitope with high specificity. The corresponding hybridoma cells not only have the ability to proliferate indefinitely but also continuously produce specific antibodies. Due to these advantages, mouse monoclonal antibodies are highly favored by researchers and serve as one of the core raw materials for vaccine and drug testing.
SERVICE ADVANTAGES
- 01SPF animal facility with strict management and transparent processes
- 05Rapid, stable antibody production via serum-free fermentation
- 02In-house adjuvants and flexible immunization options
- 06Fast-track option: antibodies and cell lines delivered in 8 weeks
- 03Enhanced SP2/0 cells and efficient electrofusion technology
- 07Skilled technical team with extensive project experience
- 04Customized screening to support downstream applications
SERVICE PROCEDURE
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01
Fusion & Screening
High-efficiency electrofusion paired with high-throughput screening, enabling the plating of fifty to one hundred 96-well plates for robust clone selection.
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02
Stable Clone Establishment
Three rounds of subcloning ensure 100% positive clones, delivering high-quality and stable hybridoma cell lines.
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03
Antibody Production
Serum-free fermentation with no animal-derived protein interference, resulting in higher specificity and reduced batch-to-batch variation.
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04
Antibody Purification & Validation
Purified using Protein A/G to obtain high-purity monoclonal antibodies, with ELISA titers exceeding 1:128,000.
SERVICE CONTENT
| No. | Items | Service Details | EDT(Week) | Deliverables |
|---|---|---|---|---|
| 1 | Antigen Preparation |
1. Customer Provides Antigen 2. Gene Universal Prepares Antigen |
4~6 | Remaining Antigen |
| 2 | Animal Immunization & Titer Testing | Immunize 5 Balb/c mice, performing 3 immunizations followed by serum collection and ELISA testing 7 days after the final immunization | 6~8 | ELISA Testing Report |
| 3 | Cell Fusion & Positive Screening | Fuse spleen cells with myeloma cells(electrofusion), culture in HAT medium, and conduct high-throughput screening | 2 | ELISA Testing Report |
| 4 | Stable Cell Line Establishment | Select 5-10 positive clones with high initial titer for subcloning, continuing until 100% positive rate is achieved in ELISA. Scale up culture and freeze down cell lines | 4 | 2-5 Stable Hybridoma Cell Lines and Corresponding Culture Supernatants |
| 5 | Small-Scale Antibody Production(Choose one) |
1. Serum-Free Fermentation: 200 ml small-scale
serum-free fermentation
2. Ascitic Fluid Production: Inject hybridoma cells into the peritoneum of 3 Balb/c mice to collect ascitic fluid. |
2 | Ascitic Fluid / Serum-Free Fermentation Broth |
| 6 | Antibody Purification & Testing | Purify the antibody from serum-free fermentation broth(or ascitic fluid)using Protein A/G, obtaining high-purity antibodies. Perform concentration, purity, and titer testing | 1 | 5-10 mg Purified Antibody with ELISA Testing Report |
| The experimental details, screening protocols, and deliverables for specific projects can be negotiated and adjusted based on the user's actual requirements. | ||||
Antigen Requirements:
1. Protein Antigen: Total amount > 5 mg, Concentration > 1 mg/ml, Purity > 90%, Molecular weight > 10 kDa, Buffer: PBS or a solution confirmed by Gene Universal as non-impactful to the experiment.
2. Peptide Antigen: Total amount > 5 mg, Purity > 90%, Buffer: PBS or a solution confirmed by Gene Universal as non-impactful to the experiment.
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