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Polyclonal Antibody Services

Polyclonal Antibody Services

OVERVIEW

Polyclonal antibodies recognize multiple epitopes, enabling precipitation and agglutination reactions. Due to their short preparation cycle and low cost, they have always been an essential tool in scientific research and an important raw material for in vitro diagnostics. Gene Universal can provide custom and modified services for rabbit and goat polyclonal antibodies against various conventional antigens. We also have extensive project experience in the development of antibodies targeting various modified.

SERVICE ADVANTAGES

  • 01SPF animal facilities, standardized management, transparent project process
  • 03Multiple purification options to ensure antibody quality
  • 02In-house developed immunoadjuvants, enhancing titer (>1:128000)
  • 04Optional rapid preparation plan, antibody delivered in 42 days

SERVICE CONTENT

Rabbit Polyclonal Antibody Production Service (Standard Antibody)

No. Items Service Details EDT Deliverable
1 Animal Immunization & Serum Collection Immunize two New Zealand White rabbits; collect serum after ELISA titer meets the required level 6~8 Weeks

· 1–2 mL pre-immune serum

· 3–5 mg antigen affinity-purified antibody

· ELISA test results (against the immunogen)

· Project Report

2 Antibody Purification Purify rabbit serum using antigen affinity; measure antibody concentration, purity, and ELISA titer 1 Week

Rabbit Polyclonal Antibody Production Service (Phosphorylated Antibody)

No. Items Service Details EDT Deliverable
1 Antigen Preparation Peptide synthesis(phosphorylated and corresponding non-phosphorylated peptides), conjugated to carrier proteins 4~6 Weeks

Remaining peptides Peptide MS and HPLC Data

2 Animal Immunization & Serum Collection Immunize two New Zealand White rabbits; collect serum after ELISA titer meets the required level 6~8 Weeks

· 1–2 mL pre-immune serum

· 1–5 mg phosphorylated antibody; 1–5 mg non-phosphorylated antibody

· ELISA results(against peptides)

· Project Report

3 Two Rounds of Affinity -Purified Antibody Affinity purify rabbit serum using phosphorylated peptide; Remove cross-reactive antibodies by adsorption with non-phosphorylated peptide; Test antibody concentration, purity, and ELISA tite 1 Week

Notes

1.Custom antibody services are also available for other modified epitopes, such as methylation, acetylation, lactylation, etc.

2.When using goats as the immunization animal, the required amount of antigen increases, and the immunization period may be extended.

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