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Single B Cell Monoclonal Antibody Service

Single B Cell Monoclonal Antibody Service

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OVERVIEW

Single B cell technology is a recently developed method for the rapid production of monoclonal antibodies. Each B cell produces only one specific antibody, containing a unique variable region sequence for both the heavy and light chains.Antigen-specific B cells are isolated from the spleen or peripheral blood of immunized rabbits. The variable regions of the IgG heavy and light chains are amplified from individual B cells using PCR, and the genes are expressed in mammalian cells to obtain functional monoclonal antibodies. This method retains the natural pairing of heavy and light chains, offering high genetic diversity, efficiency, and fully natural antibody sources. It has become a powerful strategy for fast and precise antibody development.

SERVICE HIGHLIGHTS

Beacon® is a single-cell screening platform integrating microfluidics, high-sensitivity signal detection, and optoelectronic positioning, widely used in single B cell screening for antibody discovery.

  • 01The BeaconⓇ platform enables comprehensive screening of the entire B cell population
  • 04Fully automated screening workflow
  • 021-day screening saves up to 12 weeks compared to traditional methods
  • 05Enables cross-species antibody discovery
  • 03Enables discovery of more diverse and high-affinity antibody clones

SERVICE PROCEDURE

  • 01

    AnimalImmunizationAnimal Immunization

    Protein, Peptide, and Cell-Based Immunization ( 6-8Weeks )

  • 02

    B Cell Isolation & ExpansionB Cell Isolation & Expansion

    PBMC Isolation and B Cell Expansion ( 1-2Weeks )

  • 03

    Beacon® SortingBeacon® Sorting

    B Cell Harvesting and Single B Cell Screening ( 1Day )

  • 04

    Single B Cell SequencingSingle B Cell Sequencing

    Antibody Variable Region Sequencing ( 2Weeks )

  • 05

    Antibody Expression ValidationAntibody Expression Validation

    Expression of positive sequences in mammalian cells and subsequent validation

SERVICE CONTENT

No. Items Service Details EDT Deliverables
1 Antigen Preparation 1. Provided by customer
2. Antigen preparation service(protein,peptide,mRNA,etc.) 		4~8Weeks Remaining Antigen
2 Transgenic Cell Line Construction(Optional)

1. Provided by customer

2. Construction of cell lines for immunization or membrane antibody screening

 6~8Weeks Over-expression Cell line
3 Animal Immunization Immunize 2 New Zealand white rabbits, 3–6 rounds(e.g. for rabbit mAb) 6~8Weeks Analysis Report
4 Serum Titer Testing

1. ELISA for standard antigens

2. (Optional) Flow cytometry for over-expression cell lines

3. (Optional) Functional/custom assay

 6~8Weeks Analysis Report
5 Beacon Single B CellAntibody Screening Screen 10k–30k antibody-secreting cells, export up to 192 positives; selectable strategies:
-Conventional protein/peptide binders
-Membrane protein binders
-High-affinity antibodies
-Cross-reactive binders
-Functional antibodies:neutralization, activation, internalization 		1 BD Screening Report
6 Antibody Expression Validation(One of Two Options)

1. Amplify and sequence VH/VL genes from single B cells analyze sequencing data, select top candidates for recombinant antibody expression and validation.

2. Amplify VH/VL genes from single B cells and directly clone into expression vectors for rapid antibody expression;validate supernatant or purified antibodies first, then sequence validated clones.

 2

1. VH/VL Sequences

2. Remaining Antibody

3. Antibody Validation Report

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