Custom Bacterial Expression Services

OVERVIEW

Experience unparalleled reliability with Gene Universal's E. coli protein expression service, backed by our risk-free guarantee. Our Guaranteed Bacterial Recombinant Protein Expression Service Package encompasses codon optimization, gene synthesis, subcloning, and comprehensive protein expression and purification. With our risk-free guarantee, there's no charge if the project fails. Benefit from our exceptional success rate of over 95% in E. coli expression systems. Place your order today to access top-tier services at competitive pricing in Bacterial Protein Expression.

SERVICE PROCEDURE

Gene Synthesis
& Cloning
Protein expression
evaluation and
optimization
Protein purification
pilot study
Scale-up with required
Quantity and Purity
QC & Shipping

GUARANTEED PACKAGES

Service	Tag Options	Amount	Timeline	Deliverables	SDS-PAGE Purity	Pricing	SDS-PAGE Purity	Pricing	SDS-PAGE Purity	Pricing
Risk-free Guaranteed Packages	Tag(His/GST/MBP)	1mg - 3mg	3-4 weeks	-Expression Plasmid -E.coli Expression Strain -Purified Recombinant Protein -Report & CoA	>85%	$999	>90%	$1,199	>95%	$1,499
		3mg - 5mg	3-5 weeks			$1,199		$1,399		$1,699
		5mg - 10mg	4-6 weeks			$1,399		$1,599		$1,899
	Tag-Free	1mg - 3mg	6-8 weeks			$1,399		$1,599		$1,899

1. Risk-Free Guarantee: Quantity & Purity guaranteed, or it's FREE.

2. Inclusive Pricing: Gene synthesis <2500 bp included.

3. Protein Solubility: We guarantee solubility but not bioactivity or functionality. Inclusion bodies will be refolded for solubility.

4. Tag Options: Tagged packages offer affinity purification with the final protein containing the tag. Tag-free packages use SEC and ion exchange for native folding, or fusion tag expression followed by tag removal and purification.

5. Additional Services: Endotoxin removal and specific requirements may require additional charges.
6. Quality Assurance: Purity is determined by SDS-PAGE, quantity by Bradford/BCA/A280assays. HPLC available upon request.

7. Delivery: Lyophilized powder preferred, liquid if solubility changes.

STANDARD PACKAGE

Steps	Specification	Price	EDT	Deliverables
1	Codon Optimization，Gene Synthesis	Please inquire	Varies (Please inquire)	-Plasmid(synthesized by us,2-5ug); -Optimal expression strains; -Protein(1-5 mg, SDS-PAGE purity>85%); -COA Reports
2	Expression evaluation
3	Optimization and solubility anlysis
4	Scal-up expression and purification

CASE STUDY

Case #1: Interleukin
- Challenges
  
  • Poor Solubility
  
  • Tag-free, Affinity Purification is not Applicable
  
  • Easy Polymerization
  
  • Intrachain Disulfide Bond
- Process Development
  
  • Strain screening to promote disulfide bond formation in the cytoplasm
  
  • Medium optimization, feeding strategy, especially harvest timing
  
  • IEX Capture
  
  • SEC Purification
  
  • Buffer Exchange
QC Data:
- • M:Protein Marker
  
  • R: Reducing
  
  • NR:Non-Reducing
- Yield: 350 mg/L, SDS-PAGE > 95%, SEC-HPLC > 95%
Case #2: Somatotropin
- Challenges
  
  • Inclusion Body Expression& Purification
  
  • Disulfide Bond
  
  • Easy Polymerization
  
  • Unfolding & Refolding
- Process Development
  
  • Medium optimization, fermentation process control
  
  • Dilution Refodling: dilution method/factors/speed
  
  • Screening denaturants, stabilizers, aggregation inhibitors, pH, oxidant, reductant
  
  • HIC purificatin, salt gradient elution
QC Data:
- • M:Protein Marker
  
  • R: Reducing
  
  • NR:Non-Reducing
- Yield: 500 mg/L, SDS-PAGE > 95%, SEC-HPLC > 95%

RECOMBINANT PROTEIN SERVICES FAQS

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