Product
2×GUeasy Hot Start Taq probe qPCR Master Mix

2×GUeasy Hot Start Taq probe qPCR Master Mix

This product is a 2×Mix premixed reagent, a special reagent for qPCR by probe method, which can realize multiple fluorescent quantitative PCR reactions in a single reaction well. This product contains hot-start Taq enzyme (antibody modification), which greatly improves the sensitivity and specificity of amplification. At the same time, this product has deeply optimized the multiple reaction buffer system, which can improve the amplification efficiency of the reaction, promote the effective amplification of low-concentration templates, and perform high-sensitivity Real Time PCR reactions.

Advantages Reaction System Case Study Product Specification Related Services Ordering Information

ADVANTAGES

Fast: Use a two-step procedure to quickly and accurately detect or quantify genes of interest.
Sensitive: It can effectively detect low copy number templates.
Repeatability: The optimized reaction system ensures high repeatability between experiments.

REACTION SYSTEM

Components	Volume（ul）	Final Conc.
2×GUeasy Hot Start Taq probe qPCR Master Mix	10	1×
Primer Mix（10uM）	N	0.1-0.5uM
Probe Mix（10uM）	N	50-250nM
Template	1-5	-
ddH₂0	up to 20ul	-

CASE STUDY

System: Calf thymus genome DNA template quadruple qPCR

Tested products: GU company, A company, B company

Equipment：SLAN-96S

FAM channel：GU————;A————;B————：	HEX channel：GU————;A————;B————：

ROX channel：GU————;A————;B————：	Cy5 channel：GU————;A————;B————：

PRODUCT SPECIFICATION

Product	Catalog #	Size	Unit	Price
2×GUeasy Hot Start Taq probe qPCR Master Mix	KQM3001-1	1ml	100rxn	Inquiry
	KQM3001-3	3ml	300rxn	Inquiry
	KQM3001-5	5ml	500rxn	Inquiry
	KQM3001-10	10ml	1000rxn	Inquiry
	KQM3001-30	30ml	3000rxn	Inquiry
	KQM3001-50	50ml	5000rxn	Inquiry

* More discount is available for bulk order

3._2GUeasy_Hot_Start_Taq_probe_qPCR_Master_Mix_manual_eUAHGSN.pdf

Notes

1• Be sure to mix well before use to avoid excessive air bubbles from violent shaking.

2• Primer concentration: Primer Mix contains multiple pairs of primers, usually the final concentration of each primer is 0.2μM, and can also be adjusted between 0.1-0.5μM according to the situation.

3• Probe concentration: Probe Mix contains multiple probes with different fluorescent signals, and the concentration of each probe can be adjusted between 50-250 nM according to specific conditions.

4• This product does not contain Rox reference dye. Rox reference dye: Used in Real Time PCR amplification instruments such as Applied Biosystems to correct the fluorescence signal error between wells.

5• Template dilution: qPCR is extremely sensitive, and it is recommended to dilute the template for use. If the template is a cDNA stock solution, the volume of the template should not exceed 1/10 of the total volume.

6• Reaction system: 20 μL or 50 μL is recommended to ensure the effectiveness and repeatability of target gene amplification.

7• System preparation: please prepare in the ultra-clean workbench, and use pipette tips and reaction tubes without nuclease residues; it is recommended to use pipette tips with filter elements. Avoid cross-contamination and aerosol contamination.

8• Extension：Temperature and time can be appropriately adjusted according to the Tm value of the designed primers.

Storage condition

Shipped in ice-pack, stored at -20°C, valid for two years.

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