Product
GUeasy MMLV Reverse-Transcriptase III（H-）

GUeasy MMLV Reverse-Transcriptase III（H-）

M-MLV Reverse Transcriptase (MMLV) is an RNA-mediated DNA polymerase derived from Moloney Murine Leukemia Virus. This enzyme uses RNA (synthesized from cDNA) or ssDNA as a template to make a complementary strand of DNA. Compared with wild-type M-MLV reverse transcriptase, GUeasy MMLV Reverse-Transcriptase III（H-）can effectively synthesize high-quality cDNA by removing RNase H activity through genetic modification and increasing the reverse transcription temperature to 50-60 ℃, avoiding the inhibition of cDNA synthesis by the complex secondary structure of RNA.

Advantages Operation steps Quality Control Product Specification Related Services Ordering Information

ADVANTAGES

Stability: Extremely high stability by high-standard production process.
Tolerance: For common impurities have a strong tolerance.
Compatibility: Compatible with animal, plant, virus and other templates.

OPERATION STEPS

Components	Volume（ul）
The previous reaction liquid	10
5×GUeasy MMLV Reverse-Transcriptase III buffer	4
dNTP Mix (10 mM)	1
GUeasy MMLV Reverse-Transcriptase III（200U/ul）	1
RNase inhibitor (40 U/µL)	1
RNase Free ddH2O	up to 20ul

QUALITY CONTROL

SDS-PAGE purity >98%	No exonuclease activity

No endonuclease activity	Low E.coli genome carryover

PRODUCT SPECIFICATION

Product	Catalog #	Size	Activity	Price
GUeasy MMLV Reverse-Transcriptase III（H-）	RT3003-01	100ul	20000U	Inquiry
	RT3003-02	200ul	40000U	Inquiry
	RT3003-05	500ul	100000U	Inquiry
	RT3003-1	1ml	200000U	Inquiry
	RT3003-3	3ml	600000U	Inquiry
	RT3003-5	5ml	1000000U	Inquiry

* More discount is available for bulk order

5._GUeasy_MMLV_Reverse-Transcriptase_IIIH-manual.pdf

Notes

1• When using Random Primers, incubate for 5 min at 25℃.This step may be omitted if Oligo (dT)18 or Gene Specific Primers are used.

2• Reverse transcription temperature: 42℃ is recommended. For templates with high GC content or complex secondary structure, the reverse transcription temperature can be increased to 50-55℃.

3• Heating at 85℃ for 5 min was used to inactivate reverse transcriptase.

※ Reverse transcription products can be immediately used for subsequent PCR or qPCR reaction, or can be stored at -20℃ for a short time. If long-term storage is needed, it is recommended to store them at -80℃ after packaging to avoid repeated freeze-thaw.

Storage condition

Shipped in ice-pack, stored at -20°C, valid for two years.

RELATED SERVICES

To meet your needs to the fullest extent, Gene Universal also provides

Molecular Cloning and DNA Marker Product, Protease Products, Competent Cell Products, Reagents for Molecular Diagnosis.

Online Request Submission

Have a question about sales or support? Our team is ready to help!