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Heat-labile Uracil-DNA Glycosylase

Compared with ordinary UDG enzymes, Heat-labile UDG enzymes avoid the possible residual activity of conventional UDG enzymes after inactivation, which may degrade dU-containing amplification products at room temperature. It can react at room temperature and is highly sensitive to temperature. The enzyme can be irreversibly inactivated by heating at 50°C for 10 minutes.

Ordering Information

ADVANTAGES

  • Stability: High standard production process to achieve high product stability.

  • Fast: Contaminants can be degraded during the room temperature reaction system.

  • Compatibility:excellent compatibility with various PCR and qPCR systems.

APPLICATION CASE

PCR reaction is prepared according to the following system

Components Volume(ul) Final Conc.
2×GUeasy Hot Start Taq probe qPCR Master Mix 10
Primer Mix(10uM) N 0.1-0.5uM
Probe Mix(10uM) N 50-250nM
Heat-labile Uracil-DNA Glycosylase(1U/ul) 1 0.04U/ul
Template 1-5 -
ddH2O up to 20ul -

PRODUCT SPECIFICATION

Product Catelog# Size Activity Price
Heat-labile Uracil-DNA Glycosylase HU3104-02 200ul 200U Inquiry
HU3104-05 500ul 500U Inquiry
HU3104-1 1ml 1000U Inquiry
HU3104-3 3ml 3000U Inquiry
HU3104-5 5ml 5000U Inquiry

* More discount is available for bulk order

10._Heat-labile_Uracil-DNA_Glycosylase_manual.pdf pdf

Storage condition

Shipped in ice-pack, stored at -20°C, valid for two years.

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