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SUMO Protease（His Tag）

SUMO Protease（His Tag）

SUMO Protease, also known as Ulp, is a highly active cysteine protease that recognizes the tertiary structure of the SUMO protein, thus the SUMO protein can be cut down from the recombinant fusion protein efficiently and specifically. SUMO Protease maintains a high activity in a wide range of reaction systems such as temperature (4-30 ° C), pH (5.5-9.5), and so on. The SUMO Protease is a recombinant protease that has been purified from E. coli. SUMO Protease has a His-tag which can remove by affinity chromatography.

Storage Condition Operation Method Product Specification Related Services Ordering Information

STORAGE CONDITION

It could be preserved for 2 years at -80℃, and preserved for 6 months at -20℃. It is suggested to subpackage into small volume, and avoid repeated freeze-thaw cycles.

OPERATION METHOD

Made up the follow reaction system in EP tube:
Fusion protein:1mg
SUMO Protease:10U
Mixed up and then incubated at suitable temperature, suggest reaction at 4℃ overnight.
Analyzed by SDS-PAGE after reaction, and SUMO Protease could be removed by His-tag affinity chromatography.

PRODUCT SPECIFICATION

Product names	Catalog Number	Specification	Price
SUMO Protease	MS01050	50U	$16
	MS01250	250U	$70
	MS01500	500U	$120

SUMO_Protease_Instruction.pdf

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