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T4 DNA Ligase and Quick buffer

T4 DNA ligase is often used to catalyze the ligation of double-stranded DNA ends or complementary cohesive ends, and also catalyzes the linkage between the double-stranded RNA 5'- phosphate terminal and the 3'-hydroxyl terminus. It is also possible to repair single strand breaks in double stranded DNA, RNA, or the single strand breaks in DNA/RNA hybrid double strands. All the above reactions are required to consume ATP.

Ordering Information

Advantages Reaction conditions Reaction system Related Services Ordering Information

REACTION CONDITIONS

  • Cohesive-ended connection:Reaction at 25℃ for 10~15 min;

  • Blunt-ended connection:Reaction at 25℃ for 30~40 min;

  • Cohesive-ended connection/Blunt-ended connection:Reaction at 25℃ for 120 min.

REACTION SYSTEM

Component Volume（μL）
2×Quick Buffer 5.0
Vector DNA A
Fragment DNA B
dNTP(10mM) 1μL
Enzyme 0.5~1.0
H20 Up to 10 μL

PRODUCT SPECIFICATION

Product names Catalog Specification Price
T4 DNA Ligase CL06020 20 T $25
2×Quick Buffer $25
T4 DNA Ligase CL06050 50 T $50
2×Quick Buffer $50
T4 DNA Ligase CL06100 100 T $90
2×Quick Buffer $90
T4_DNA_Quick_Ligation_Kit_Instruction.pdf pdf

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