Uracil-DNA Glycosylase

The principle of action of UDG enzyme is to selectively hydrolyze and break the uridine glycosidic bond of double-stranded or single-stranded DNA containing dU. Replace dTTP with dUTP, so that all PCR products contain dU DNA strands. Adding an incubation step before the start of PCR, the UDG enzyme can degrade the uracil bases in the existing U-DNA pollutants in the reaction system, and break the DNA strands under subsequent denaturing conditions, eliminating the pollution caused by aerosols Amplification, so as to ensure the specificity and accuracy of the amplification results. The UDG enzyme was inactivated at the high temperature of 95°C at the beginning of PCR, and it would no longer degrade the newly amplified product U-DNA.