Custom Stable Cell Lines for Gene Overexpression and Knockdown

OVERVIEW

Stable cell lines are widely used in gene function research and the development of animal disease models, as they help overcome the variability and instability of transient transfection efficiency. Gene Universal offers a mature and reliable platform for the construction of stable cell lines for both gene overexpression and gene knockdown. Our stable overexpression cell lines are generated using lentiviral transduction combined with antibiotic selection, enabling the establishment of stable cell lines across a wide range of cell types.

SERVICE HIGHLIGHTS

Permanent Integration

Lentiviral transduction enables stable integration of the exogenous gene into the host cell genome and all subsequent daughter cells.
Broad Host Range

Lentiviruses are compatible with most mammalian cell types, allowing efficient transduction of target cells.
Rapid Turnaround

Gene Universal offers a one-stop solution covering primer synthesis, gene synthesis, viral packaging, and cell engineering, enabling fast and efficient project delivery.

SERVICE PROCEDURES

SERVICE DETAILS

Service Type	Service Name	Deliverables	Turnaround time(BD)	Price(USD)	Remarks
Overexpression	Overexpression cell pool(qPCR Validation)	Target,Control ,and Wild-type cells, >10^6 cells/tube *2 tubes for each type of cell	40	1800	Includes gene construction + Lentivirus packaging (gene≤3kbp)
Overexpression	Overexpression cell pool(qPCR and Western blot Validation)	Target,Control ,and Wild-type cells, >10^6 cells/tube *2 tubes for each type of cell	50	2160	Includes gene construction + Lentivirus packaging (gene≤3kbp)
Overexpression	Overexpression cell line(qPCR Validation)	Target,Control ,and Wild-type cells, >10^6 cells/tube *2 tubes for each type of cell	70	2700	Includes gene construction + Lentivirus packaging (gene≤3kbp)
Overexpression	Overexpression cell line(qPCR and Western blot Validation)	Target,Control ,and Wild-type cells, >10^6 cells/tube *2 tubes for each type of cell	80	3060	Includes gene construction + Lentivirus packaging (gene≤3kbp)
Overexpression	Overexpression cell pool(qPCR Validation)	Target,Control ,and Wild-type cells, >10^6 cells/tube *2 tubes for each type of cell	20	1080	Customer provides Lentivirus
Overexpression	Overexpression cell pool(qPCR and Western blot Validation)	Target,Control ,and Wild-type cells, >10^6 cells/tube *2 tubes for each type of cell	30	1440	Customer provides Lentivirus
Overexpression	Overexpression cell line(qPCR Validation)	Target,Control ,and Wild-type cells, >10^6 cells/tube *3 tubes for each type of cell	50	1980	Customer provides Lentivirus
Overexpression	Overexpression cell line(qPCR and Western blot Validation)	Target,Control ,and Wild-type cells, >10^6 cells/tube *4 tubes for each type of cell	60	2340	Customer provides Lentivirus
RNAi	RNAi cell pool(qPCR Validation)	Target,Control ,and Wild-type cells, >10^6 cells/tube *3 tubes for each type of cell	40	1500	Includes gene construction + Lentivirus packaging
RNAi	RNAi cell pool(qPCR and Western blot Validation)	Target,Control ,and Wild-type cells, >10^6 cells/tube *3 tubes for each type of cell	50	1800	Includes gene construction + Lentivirus packaging
RNAi	RNAi cell line(qPCR Validation)	Target,Control ,and Wild-type cells, >10^6 cells/tube *3 tubes for each type of cell	70	2250	Includes gene construction + Lentivirus packaging
RNAi	RNAi cell line(qPCR and Western blot Validation)	Target,Control ,and Wild-type cells, >10^6 cells/tube *3 tubes for each type of cell	80	2550	Includes gene construction + Lentivirus packaging

Note: Cells, relevant detection antibodies, and any special culture media or special FBS must be provided by the customer.

CASE STUDY

KYSE-150-Overexpression Cell pool

95KD Flag

37KD GAPDH

WTCtrlOE

Figure: Fluorescence imaging and Western blot analysis demonstrating stable overexpression of the target gene in KYSE-150 cells.

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