Large Scale Antibody Production

OVERVIEW

Manufacturing antibodies or proteins on a large scale is essential for various applications such as in vitro studies, in vivo animal experiments, and pre-clinical trials. Gene Universal provides a comprehensive large-scale production service, delivering recombinant antibodies or proteins ranging from milligrams to grams within weeks.With over a decade of expertise in antibody production, discovery, and optimization, Gene Universal has solidified its position as a trusted CRO in custom antibody manufacturing. Committed to excellence, we deliver high-quality products to biotechnology and pharmaceutical companies worldwide.

SERVICE HIGHLIGHTS

• 
  Continuous manufacturing
  24/7 operation enables large-scale
  and rapid production
• 
  Large Capacity
  Culture volumes vary from
  shake flasks to bioreactors
• 
  High Quality
  SEC-HPLC > 95%,
  endotoxin < 1 EU/mg

SERVICE DETAILS: 2-4 WEEKS

• 01

  Gene Synthesis & Subcloning

  · Codon Optimization and Gene Synthesis

  · Subcloning

  · Large-scale Plasmid Preparation

• 02

  Expression and Purification

  · Transient Transfection of HEK293/CHO Cells

  · Antibody Purification

• 03

  QC Analysis

  · SDS-PAGE > 95%

  · SEC-HPLC > 95%

  · Endotoxin < 1 EU/mg

  · Biacore (optional)

• 04

  Deliverables

  · Purified Antibodies

  · Expression Plasmids Contain GOI

  · COA Report

FLEXIBLE TO DIFFERENT SCALES

CASE STUDY

Human lgG (CHO-S)

M: Protein Marker

R: Reducing

N-R: Non-Reducing

Yield: 413 mg/L, SDS-PAGE > 95%, SEC-HPLC>95%

VHH with Fc tag (293F)

M: Protein Marker

R: Reducing

N-R: Non-Reducing

Yield: 308 mg/L, SDS-PAGE > 95%, SEC-HPLC>95%

ScFv with 6x His tag (CHO-S)

M: Protein Marker

R: Reducing

N-R: Non-Reducing

Yield: 308 mg/L, SDS-PAGE > 95%, SEC-HPLC>95%