Mammalian Expression
  • Overview
  • Service Descriptions
  • Service Procedure
  • Service Options

The mammalian cell expression system, known for its intricate glycosylation processes, is essential for achieving precise post-translational modifications and correct protein folding. Particularly crucial for downstream applications, transient expression in mammalian cells is widely utilized in the development and production of recombinant protein drugs, notably therapeutic recombinant monoclonal antibodies.

Gene Universal's mammalian expression services streamline the production and delivery of high-quality proteins and recombinant antibodies (rAbs), ranging from microgram to gram quantities, in CHO or HEK293 cells. With guaranteed protein quantity and purity, we leverage proprietary transient mammalian expression technology to optimize cellular pathways, resulting in exceptional protein and antibody yields (up to 3 g/L).

Our guaranteed mammalian protein expression packages encompass gene synthesis and subcloning, progressing seamlessly through protein expression and purification using our proprietary mammalian protein expression and purification technologies. Trust Gene Universal to propel your research forward with reliable and efficient mammalian protein expression solutions.

Service Descriptions

One-Stop Solution: Seamless integration from gene synthesis to protein and antibody production.

Inclusive Gene Synthesis: No additional cost for gene synthesis.

Competitive Pricing: Cost-effective solutions tailored to your scaling needs.

Swift Turnaround: Expedited service with turnaround times as fast as 2 weeks.

Ultra-Low Endotoxin Levels: as low as 1 EU/mg.

No Setup Fees: Transparent pricing without setup fees.

Flexible Packages: Customize your package according to your specific requirements.

Service Procedure

  • Protein


  • Gene Synthesis

  • Plasmid Prep

  • Transient Transfection

    & Expression

  • Purification & QC

  • Fast


Service Options

Mammalian system service type

Service Step Service Description Timeline Deliverables
Gene Synthesis

Codon Optimization & Gene Synthesis


Plasmid Preparation

2 to 3 weeks

Expression Plasmid Contain GOI

Purified Recombinant Protein (SDS-PAGE & SEC-HPLC Detection)

COA Report

Pilot Study

Pilot Protein Expression

Pilot Protein Purification

QC Analysis

Scale-up Production

Large-scale Protein Expression


QC Analysis

3 to 4 weeks

Purified Recombinant Protein (SDS-PAGE & SEC-HPLC Detection)

COA Report

Terms and Conditions

1. Flexibility in Payment: Begin your project according to your preferences, and we'll charge only for each step as it progresses, ensuring transparency and flexibility.

2. Diverse Purification Methods: Choose from affinity purification, ion exchange purification, hydrophobic purification, and SEC purification. Keep in mind that the expression system selected will impact the final protein's purity and activity, ensuring optimal outcomes tailored to your needs.

Case Study

● Fibroblast growth factor 21 with N-Fc tag (293F)


M: Protein Marker

Lane 1: Reducing

Lane 2: Non-Reducing


SDS-PAGE > 95%, SEC-HPLC > 95%

● Scfv with His tag (CHO-S)


M: Protein Marker

Lane 1: Reducing

Lane 2: Non-Reducing


SDS-PAGE > 95%, SEC-HPLC > 95%

How to Order?


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