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Mammalian Expression

OVERVIEW

The mammalian cell expression system, known for its intricate glycosylation processes, is essential for achieving precise post-translational modifications and correct protein folding. Particularly crucial for downstream applications, transient expression in mammalian cells is widely utilized in the development and production of recombinant protein drugs, notably therapeutic recombinant monoclonal antibodies.

Gene Universal's mammalian expression services streamline the production and delivery of high-quality proteins and recombinant antibodies (rAbs), ranging from microgram to gram quantities, in CHO or HEK293 cells. With guaranteed protein quantity and purity, we leverage proprietary transient mammalian expression technology to optimize cellular pathways, resulting in exceptional protein and antibody yields (up to 3 g/L).

Our guaranteed mammalian protein expression packages encompass gene synthesis and subcloning, progressing seamlessly through protein expression and purification using our proprietary mammalian protein expression and purification technologies. Trust Gene Universal to propel your research forward with reliable and efficient mammalian protein expression solutions.

SERVICE DESCRIPTIONS

  • 01. One-Stop Solution:

    Seamless integration from gene synthesis to protein and antibody production.

  • 05. Ultra-Low Endotoxin Levels:

    As low as 1 EU/mg.

  • 02. Inclusive Gene Synthesis:

    No additional cost for gene synthesis.

  • 06. No Setup Fees:

    Transparent pricing without setup fees.

  • 03. Competitive Pricing:

    Cost-effective solutions tailored to your scaling needs.

  • 07. Flexible Packages:

    Customize your package according to your specific requirements.

  • 04. Swift Turnaround

    Expedited service with turnaround times as fast as 2 weeks.

SERVICE PROCEDURE

  • Protein_Sequence viral_delivery_left

    Protein
    Sequence

  • Gene_Synthesis_1 viral_delivery_left

    Gene Synthesis

  • Plasmid_Prep viral_delivery_left

    Plasmid Prep

  • Transient_Transfection__Expression viral_delivery_left

    Transient Transfection
    & Expression

  • Purification__QC viral_delivery_left

    Purification & QC

  • Fast_Delivery

    Fast
    Delivery

SERVICE DETAILS

Service Step Service Description Timeline Deliverables
Gene Synthesis

· Codon Optimization & Gene Synthesis

· Subcloning

· Plasmid Preparation

2 to 3 weeks

· Expression Plasmid Contain GOI

· Purified Recombinant Protein (SDS-PAGE & SEC-HPLC Detection)

· COA Report

Pilot Study

· Pilot Protein Expression

· Pilot Protein Purification

· QC Analysis

Scale-up Production

· Large-scale Protein Expression

· Purification

· QC Analysis

3 to 4 weeks

· Purified Recombinant Protein (SDS-PAGE & SEC-HPLC Detection)

· COA Report

CASE STUDY

Fibroblast growth factor 21 with N-Fc tag (293F)

Fibroblast

M: Protein Marker

Lane 1: Reducing

Lane 2: Non-Reducing

SDS-PAGE

SDS-PAGE > 95%, SEC-HPLC > 95%

Scfv with His tag (CHO-S)

tag

M: Protein Marker

Lane 1: Reducing

Lane 2: Non-Reducing

Scfv

SDS-PAGE > 95%, SEC-HPLC > 95%

RECOMBINANT PROTEIN SERVICES FAQs

1. What expression systems do you offer for recombinant protein production?
2. What is the typical yield for recombinant protein production?
3. Can you produce proteins with post-translational modifications?
4. Do you offer purification services for recombinant proteins?
5. How do you handle challenging proteins that are difficult to express or purify?
6. What is the level of purity that can be achieved for recombinant proteins?
7. Do you offer protein characterization services along with production?

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