Custom Adenovirus Packaging Services & Vector Production

OVERVIEW

Recombinant adenovirus (Ad) is a replication-defective adenovirus vector system. Adenovirus packaging relies on cell lines expressing E1 genes, such as HEK-293 and HEK-293A, as the adenoviral packaging is defective in the early transcription of replication genes E1 and E3 (DE1, DE3), with the E3 gene being non-essential for viral production.

The widely utilized Adenovirus vectors are predominantly derived from human adenovirus type 5 (Ad5), featuring a linear double-stranded DNA genome approximately 36 Kb in length. Ad5 vectors offer several notable advantages: they possess a broad infection range, with nearly all cell lines and tissues susceptible to infection, achieving efficiencies as high as 100%. This efficacy surpasses that of other viral vector tools and liposome transfection methods. Additionally, Ad5 vectors do not integrate their genome into host cell genes, mitigating the risks associated with gene mutation and integration. As a result, recombinant adenovirus finds extensive applications in gene therapy, fundamental life science research, and various other fields.

Gene Universal's adeasy adenovirus packaging system (Ad5 type) offers distinct advantages compared to the admax system. Notably, it facilitates easier detection of false positives of linearized plasmid recombination in the pronucleus. Additionally, the linearized adenovirus plasmid can be utilized in cell lines expressing E1, such as HEK-293 and HEK-293A. Our adenovirus vectors meet the rigorous requirements for both cell infection and in vivo experiments, ensuring reliable results for your research endeavors.

ADVANTAGES

High infection efficiency and expression levels enable adenovirus vectors to infect nearly all types of cells.
High titer ensures that purified adenovirus is well-suited for in vivo experiments.
Ease of amplification significantly reduces costs.
Non-integration into the host cell genome ensures high safety.
Adenovirus vectors have the largest capacity among viral vectors, ideal for carrying large exogenous gene fragments.

SERVICE DETAILS

Scale	Package	Titer	Turnaround	Price
Medium	Customized Over-expression Adenovirus ( Gene ≤ 3kb )	≥ 2x10¹⁰ PFU/ml	55 BD	$679
	RNAi / sgRNA Adenovirus-single target( sgRNA only , no Cas9 gene )	≥ 2x10¹⁰ PFU/ml	45 BD	$599
	RNAi / sgRNA Adenovirus-three targets( sgRNA only, no Cas9 gene )	≥ 2x10¹⁰ PFU/ml	45 BD	$1,529
	sgRNA-Cas9 Adenovirus( sgRNA+Cas9 )	≥ 2x10¹⁰ PFU/ml	55 BD	$679
	miRNA-up / down-Adenovirus	≥ 2x10¹⁰ PFU/ml	45 BD	$599
Large	Customized Over-expression Adenovirus ( Gene ≤ 3kb )	≥ 10¹¹ PFU/ml	60 BD	$1,219
	RNAi / sgRNA Adenovirus-single target( sgRNA only , no Cas9 gene )	≥ 10¹¹ PFU/ml	50 BD	$1,299
	sgRNA-Cas9 Adenovirus( sgRNA+Cas9 )	≥ 10¹¹ PFU/ml	60 BD	$1,449

* Listed price includes RNA synthesis. For Customized Over-expression Adenovirus, a $0.12/bp gene synthesis fee applies.

* Turnaround time includes plasmid construction and preparation.

DELIVERABLES

Product	Adenovirus Packaging Services
Deliverables	Custom Adenovirus, delivered to specification: Medium Scale: ≥ 2x10¹⁰ PFU/ml, 5 x 200 μl Large Scale: ≥ 10¹¹ PFU/ml, 5 x 200 μl
	Free: Control virus Medium Scale: ≥ 2x10¹⁰ PFU/ml, 5 x 200 μl Large Scale: ≥ 10¹¹ PFU/ml, 5 x 200 μl
	Polybrene,10 mg/ml, 1 x 200 μl (Free)
Transport/storage	Dry ice/-80 ° C, avoid repeated freezing and thawing

Transport/storage：

Adenovirus will be shipped in buffer on dry ice.

Upon receipt, store Adenovirus at -80℃.

Validity period: 1 year.

Avoid repeated freezing and thawing to maintain Adenovirus titer.

CASE STUDY

Fluorescence microscopy imaging performed after cell infection

HN1 adenoviral infection of VERO cells (bright field)

HN1 adenoviral infection of VERO cells (green fluorescence)

ADENOVIRUS PACKAGING FAQs

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