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Bacterial Expression

Bacterial Expression

Limited Time Only 20% off Until 12/31/2024

OVERVIEW

Experience unparalleled reliability with Gene Universal's E. coli protein expression service, backed by our risk-free guarantee. Our Guaranteed Bacterial Recombinant Protein Expression Service Package encompasses codon optimization, gene synthesis, subcloning, and comprehensive protein expression and purification. With our risk-free guarantee, there's no charge if the project fails. Benefit from our exceptional success rate of over 95% in E. coli expression systems. Place your order today to access top-tier services at competitive pricing in Bacterial Protein Expression.

SERVICE PROCEDURE

  • _Cloning viral_delivery_left

    Gene Synthesis
    & Cloning

  • Protein_expression_evaluation viral_delivery_left

    Protein expression
    evaluation and
    optimization

  • Protein_purification_pilot_study viral_delivery_left

    Protein purification
    pilot study

  • Scale-up_with_required viral_delivery_left

    Scale-up with required
    Quantity and Purity

  • _Shipping

    QC & Shipping

Exclusive Limited-Time Discounts Available Now!

Service Type Current Discount Effective Date
Guaranteed Packages 20% 03/01/2025-06/30/2025

GUARANTEED PACKAGES

Risk-free E.coli Protein
Service Packages
Description
Amount
Timeline
Purity
Pricing
Risk-free Guaranteed Packages
(Tagged:His/MBP/GST)

‐Gene Synthesis & cloning

‐Expression pilot study

‐Tag removal(optional)

‐Guaranteed quantity of purified protein

‐QC:SDS-PAGE and Western Blot(Tagged)

3mg(Guaranteed)
4-6 weeks
(From gene synthesis
to purified proteins)
85%
US$1,450
90%
US$1,850
95%
US$2,250
5mg(Guaranteed)
85%
US$1,950
90%
US$2,350
95%
US$2,750
10mg(Guaranteed)
85%
US$2,450
90%
US$2,850
95%
US$3,250
Other Quantity
Quote
Risk-free Guaranteed Packages
(Tag-free)
3mg(Guaranteed)
85%
US$2,850
90%
US$3,850
5mg(Guaranteed)
85%
US$3,950
90%
US$4,950
10mg(Guaranteed)
85%
US$6,050
90%
US$7,050
Other Quantity
Quote

  • 1. Risk-Free Guarantee: Quantity & Purity guaranteed, or it's FREE.

    2. Inclusive Pricing: Gene synthesis <3000 bp included.

    3. Protein Solubility: We guarantee solubility but not bioactivity or functionality. Inclusion bodies will be refolded for solubility.

    4. Tag Options: Tagged packages offer affinity purification with the final protein containing the tag. Tag-free packages use SEC and ion exchange for native folding, or fusion tag expression followed by tag removal and purification.

    5. Additional Services: Endotoxin removal and specific requirements may require additional charges.

  • 6. Quality Assurance: Purity is determined by SDS-PAGE, quantity by Bradford/BCA/A280assays. HPLC available upon request.

    7. Delivery: Lyophilized powder preferred, liquid if solubility changes.

CUSTOM PACKAGE(CHARGED BY STEPS)

Steps
Specification
Timeline
Price
Deliverables

1. Gene synthesis or Subcloning, Plasmid Prep

A).Gene synthesis sub cloning into expression vector

Varies
(Please inquire)
$0.12/bp (min:$69/gene)

‐Desired quantity of purified,
soluble protein

‐Plasmid(synthesized by us,2-5ug)

‐QC data

B).Or provide your expression constructs, we’ll sub-clone your gene into our expression vector

$49/construct
2.Expression Pilot Study
& Optimization

A).Protein expression evaluation: Transformation and 37℃ inducing condition (5 cloning)

B).Optimization and solubility anlysis: Two temperature (37℃ and 15℃); Two IPTG concetration(0.2mM and 1mM IPTG)

$300
3.Protein Purification
1L expression and purification, protein (purity>85%)
$400
4.Scale-up and purification
Large scale expression & purification at your desired quantity (mg to g)
Quote
5.Tag removal and endotoxin removal and other steps(Optional)
Perform tag removal ,endotoxin removal,higher purity and other steps as needed per your request
Quote
6.QC testing
SDS-PAGE and Western Blot(tagged protein only)
/

CASE STUDY

  • Case #1: Interleukin

    • Challenges

      • Poor Solubility

      • Tag-free, Affinity Purification is not Applicable

      • Easy Polymerization

      • Intrachain Disulfide Bond

    • Process Development

      • Strain screening to promote disulfide bond formation in the cytoplasm

      • Medium optimization, feeding strategy, especially harvest timing

      • IEX Capture

      • SEC Purification

      • Buffer Exchange

    QC Data:

    • Protein

      • M:Protein Marker

      • R: Reducing

      • NR:Non-Reducing

    • Yield

      Yield: 350 mg/L, SDS-PAGE > 95%, SEC-HPLC > 95%

  • Case #2: Somatotropin

    • Challenges

      • Inclusion Body Expression& Purification

      • Disulfide Bond

      • Easy Polymerization

      • Unfolding & Refolding

    • Process Development

      • Medium optimization, fermentation process control

      • Dilution Refodling: dilution method/factors/speed

      • Screening denaturants, stabilizers, aggregation inhibitors, pH, oxidant, reductant

      • HIC purificatin, salt gradient elution

    QC Data:

    • Protein

      • M:Protein Marker

      • R: Reducing

      • NR:Non-Reducing

    • Yield

      Yield: 500 mg/L, SDS-PAGE > 95%, SEC-HPLC > 95%

RECOMBINANT PROTEIN SERVICES FAQS

1. What expression systems do you offer for recombinant protein production?
2. What is the typical yield for recombinant protein production?
3. Can you produce proteins with post-translational modifications?
4. Do you offer purification services for recombinant proteins?
5. How do you handle challenging proteins that are difficult to express or purify?
6. What is the level of purity that can be achieved for recombinant proteins?
7. Do you offer protein characterization services along with production?

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