Experience unparalleled reliability with Gene Universal's E. coli protein expression service, backed by our risk-free guarantee. Our Guaranteed Bacterial Recombinant Protein Expression Service Package encompasses codon optimization, gene synthesis, subcloning, and comprehensive protein expression and purification. With our risk-free guarantee, there's no charge if the project fails. Benefit from our exceptional success rate of over 95% in E. coli expression systems. Place your order today to access top-tier services at competitive pricing in Bacterial Protein Expression.
Gene Synthesis
& Cloning
Protein expression evaluation
and optimization
Protein purification
pilot study
Scale-up with required
Quantity and Purity
QC & Shipping
Exclusive Limited-Time Discounts Available Now!
Service Type | Current Discount | Effective Date |
---|---|---|
Guaranteed Packages | 20% | 01/01/2025-03/31/2025 |
Risk-free E.coli Protein
Service Packages |
Description
|
Amount
|
Timeline
|
Purity
|
Pricing
|
---|---|---|---|---|---|
Risk-free Guaranteed Packages
(Tagged:His/MBP/GST) |
‐Gene Synthesis & cloning
‐Expression pilot study ‐Scale-up and purification ‐Tag removal(optional) ‐Guaranteed quantity of purified protein ‐QC:SDS-PAGE and Western Blot(Tagged) |
3mg(Guaranteed)
|
4-6 weeks
(From gene synthesis to purified proteins) |
85%
|
US$1,450
|
90%
|
US$1,850
|
||||
95%
|
US$2,250
|
||||
5mg(Guaranteed)
|
85%
|
US$1,950
|
|||
90%
|
US$2,350
|
||||
95%
|
US$2,750
|
||||
10mg(Guaranteed)
|
85%
|
US$2,450
|
|||
90%
|
US$2,850
|
||||
95%
|
US$3,250
|
||||
Other Quantity
|
Quote
|
||||
Risk-free Guaranteed Packages
(Tag-free) |
3mg(Guaranteed)
|
85%
|
US$2,850
|
||
90%
|
US$3,850
|
||||
5mg(Guaranteed)
|
85%
|
US$3,950
|
|||
90%
|
US$4,950
|
||||
10mg(Guaranteed)
|
85%
|
US$6,050
|
|||
90%
|
US$7,050
|
||||
Other Quantity
|
Quote
|
1. Risk-Free Guarantee: Quantity & Purity guaranteed, or it's FREE.
2. Inclusive Pricing: Gene synthesis <3000 bp included.
3. Protein Solubility: We guarantee solubility but not bioactivity or functionality. Inclusion bodies will be refolded for solubility.
4. Tag Options: Tagged packages offer affinity purification with the final protein containing the tag. Tag-free packages use SEC and ion exchange for native folding, or fusion tag expression followed by tag removal and purification.
5. Additional Services: Endotoxin removal and specific requirements may require additional charges.
6. Quality Assurance: Purity is determined by SDS-PAGE, quantity by Bradford/BCA/A280assays. HPLC available upon request.
7. Delivery: Lyophilized powder preferred, liquid if solubility changes.
Steps
|
Specification
|
Timeline
|
Price
|
Deliverables
|
---|---|---|---|---|
1.Gene synthesis or Subcloning,
Plasmid Prep |
A).Gene synthesis sub cloning into expression vector
|
Varies
(Please inquire) |
$0.12/bp (min:$69/gene)
|
‐Weekly progress reports
‐Desired quantity of purified, ‐Plasmid(synthesized by us,2-5ug) ‐QC data |
B).Or provide your expression constructs, we’ll sub-clone your gene into our expression vector
|
$49/construct
|
|||
2.Expression Pilot Study
& Optimization |
A).Protein expression evaluation:
Transformation and 37℃ inducing condition (5 cloning)
B).Optimization and solubility anlysis:
Two temperature(37℃ and 15℃);
Two IPTG concetration(0.2mM and 1mM IPTG)
|
$300
|
||
3.Protein Purification
|
1L expression and purification, protein (purity>85%)
|
$400
|
||
4.Scale-up and purification
|
Large scale expression & purification at your desired quantity (mg to g)
|
Quote
|
||
5.Tag removal and endotoxin removal and other steps(Optional)
|
Perform tag removal ,endotoxin removal,higher purity and other steps as needed per your request
|
Quote
|
||
6.QC testing
|
SDS-PAGE and Western Blot(tagged protein only)
|
/ |
Case Study
Case #1: Interleukin
Challenges
Process Development
QC Data:
M:Protein Marker
R: Reducing
NR:Non-Reducing
Yield: 350 mg/L, SDS-PAGE > 95%, SEC-HPLC > 95%
Case #2: Somatotropin
Challenges
Process Development
QC Data:
M:Protein Marker
R: Reducing
NR:Non-Reducing
Yield: 500 mg/L, SDS-PAGE > 95%, SEC-HPLC > 95%
How to Order?