Custom IVT mRNA Synthesis

OVERVIEW

Exogenous gene expression in target cells typically necessitates the introduction of viral or non-viral vectors containing exogenous DNA fragments. However, traditional methods involving the direct introduction of exogenous DNA into the human body pose safety concerns in clinical applications. In contrast, in vitro transcribed (IVT) mRNA offers inherent safety advantages due to its non-infectious nature, lack of genomic integration, and natural degradation in cells. Consequently, IVT mRNA is increasingly favored as a tool for gene therapy and novel vaccine research.

Gene Universal boasts a professional and experienced technical team dedicated to custom IVT mRNA synthesis, offering a comprehensive one-stop service from gene synthesis, plasmid preparation, linearized plasmid to IVT mRNA synthesis. Our IVT mRNA ranges in length from 100nt to 5000nt and can be tailored and optimized for various downstream applications. These include template optimization, coding region codon optimization, nucleoside modification, and post-transcriptional modification. IVT mRNA finds applications in diverse research fields such as cell transfection, embryo injection, biopharmaceuticals, and vaccine development.

SERVICE FEATURES

Free codon optimization enhances mRNA translation efficiency.
Optimized vector for increased IVT efficiency.
Customized cap and tail options: 5’ cap (Uncapped, Cap0, Cap1) and 3’ PolyA Tail (No Tail, 120A Tail, customized) to boost mRNA stability and translation efficiency.
Enhance mRNA translation efficiency and immune evasion performance with modifications like 5-Methyl-CTP and Pseudo-UTP.
Tailored quality control plan with customized QC projects to meet specific needs.
One-stop service from DNA template synthesis to IVT mRNA Production.
Industry-leading turnaround for rapid delivery.

SERVICE OPTIONS

Services Step	Service Content	Turnaround Time	Price
1. Gene synthesis, Plasmid Preparation	Gene synthesis, Plasmid construction	2-3 weeks	Inquiry
1. Gene synthesis, Plasmid Preparation	Plasmid preparation	2-3 weeks
2. IVT mRNA Synthesis	Linearized plasmid	3-4weeks
2. IVT mRNA Synthesis	IVT, add 5'cap and 3'PolyA tail, modification	3-4weeks
3. Quality Control	The default QC+extra QC item(Customized)	Inquiry

QC ITEM

QC	Item	Test Method	Specification
Default	mRNA Length	Agarose gel electrophoresis	Expected size band detected
	mRNA Sequence	Sanger sequencing	Correct DNA template
	Concentration	Nano drop or Qubit	1μg/μl(Default)
	Appearance	Visual inspect	Clear, no visible particles
	A260/280	Nano drop	2.0-2.2
	Purity	HPLC	≥90%
Extra(optional)*	Residual DNA template	qPCR	≤ 10pg/ug
	Endotoxin level	Gel-TAL	≤0.005 EU/μg
	Residual Protein	ELISA or Qubit	≤ 1%
	Bioburden	Plate	48h sterile spots