Gene Universal

OVERVIEW

Gene Universal now offers high-quality, sequence-verified single-stranded DNA (ssDNA), ideal for maximizing the editing efficiency of your CRISPR-mediated gene knock-in, in-vitro transcription, and other experiments. With high editing efficiency and lower off-target integration, ssDNA is considered the optimal CRISPR Homology Directed Repair (HDR) template for creating gene knock-ins to develop transgenic animal models and cell lines.

Advantages of leveraging ssDNA as CRISPR-mediated gene knock-in HDR Templates.

01. Lower cellular toxicity

02. Reduced off-target integration

03. High efficiency

04. Increased editing accuracy

SERVICE FEATURES

01. Sanger sequencing verifies sequence accuracy.
02. Non-detectable levels of dsDNA and minimal DNA base damage.
03. Free gene template storage, supporting future reorders.
04. Expertise in synthesizing difficult genes as ssDNA templates.
05. Industry-leading turnaround for rapid delivery.
06. Yield flexibility: Choose from 3, 5, 10, or more µg of lyophilized fragments.

SERVICE DETAILS

Length (Nucleotides)	Quantity	Price	Turnaround Time (Business Days)
151-500	3 ug	$350	10-12 Business Days
	5 ug	$500
	10 ug	$750
	>10 ug	Inquiry	Inquiry
501-3000	3 ug	$0.75/nt	15-18 Business Days
	5 ug	$0.9/nt
	10 ug	$1.2/nt
	>10 ug	Inquiry	Inquiry
3001-5000	Inquiry	Inquiry	Inquiry

* Please note: Shipped as lyophilized powder. Enjoy 50% off complementary sequence for every order. For complex sequences (GC/AT rich, poly structure, hairpin, repeats), please request a quote.

APPLICATIONS OF ssDNA SYNTHESIS

1. CRISPR-mediated gene knock-in, replacement, or correction

01. Double Stranded Breaks (DSBs)

02. Guide RNA (gRNA)

03. Protospacer Adjacent Motif (PAM)

04. Non-Homologous End-joining (NHEJ)

05. Homology Directed Repair (HDR)
2. In vitro transcription

Transcription initiates DNA-based gene expression, converting DNA segments into RNA, particularly mRNA, through RNA polymerase enzymes. In vitro transcription (IVT) utilizes RNA polymerases (T7, T3, or SP6) and DNA templates to transcribe RNA molecules. Templates for IVT include double-stranded DNA fragments, circular plasmid DNA, and single-stranded donor oligonucleotides (ssODN) or single-stranded single-stranded DNA (ssDNA).