
OVERVIEW
Site-directed mutagenesis introduces changes to DNA fragments by PCR mutagenesis, including deletion, insertion and point mutation, to enable studies of gene regulation, DNA-protein interactions, protein structure/function, enzyme active sites and novel protein. Maximize your time for research,simply design your mutations and leave the rest to us. With Gene Universal, expect 100% accuracy in your mutant constructs, empowering your discoveries.
FEATURES
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100% Accuracy: Sequence-verified mutant constructs via Sanger sequencing.
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Unlimited Sites: Introduce mutations anywhere with our advanced technology.
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Competitive Prices: Starting from $99 per mutant.
SERVICE DESCRIPTIONS
Enhance your research efficiency by partnering with us for seamless mutagenesis and gene synthesis solutions.
Here's what we need for your custom DNA template:
· DNA Template: Ship us your DNA template plasmid or bacterial culture. We recommend 4 μg of template DNA for optimal results.
· Sequence Details: Provide the complete sequence of the template, including the target gene and its vector.
· Mutation Specifications: Clearly outline your mutation requirements.
· Maps and Antibiotic Resistance: Include maps and antibiotic resistance details of both the template and destination vectors.
SERVICE PROCEDURE
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Materials provided
by customer -
QC identification
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Design and
optimize
the primer -
Cloned into original
vector or appointed vector -
Sequencing
& Validation -
Delivery
SERVICE OPTIONS
Length | Mutated Site | EDT | Price |
---|---|---|---|
< 3000 bp | One | 5-8 Business Days | $99/mutation |
Two | 10-14 Business Days | $149/mutation | |
Three | 15-20 Business Days | $199/mutation | |
3000 bp-8000 bp | One | 5-8 Business Days | $129/mutation |
Two | 10-14 Business Days | $179/mutation | |
Three | 15-20 Business Days | $229/mutation | |
> 8000 bp | One | Inquiry | Inquiry |
Two | Inquiry | Inquiry | |
Three | Inquiry | Inquiry |
One mutation is defined as any combination of base changes within a 30-base frame.
STANDARD DELIVERABLES
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2-5μg of lyophilized plasmid containing target gene
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Sequence chromatograms covering your gene (electronic)
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Construct map for the plasmid (electronic)
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Quality assurance certificate
Request A Quote
Download and fill out the order form below Order form
Please send e-mail to us for quotation sales@geneuniversal.com
Online Request Submission
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