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Site-directed Mutagenesis

Site-directed Mutagenesis

OVERVIEW

Site-directed mutagenesis introduces changes to DNA fragments by PCR mutagenesis, including deletion, insertion and point mutation, to enable studies of gene regulation, DNA-protein interactions, protein structure/function, enzyme active sites and novel protein. Maximize your time for research,simply design your mutations and leave the rest to us. With Gene Universal, expect 100% accuracy in your mutant constructs, empowering your discoveries.

FEATURES

  • site_directed_mutagenesis_1

    100% Accuracy: Sequence-verified mutant constructs via Sanger sequencing.

  • site_directed_mutagenesis_2

    Unlimited Sites: Introduce mutations anywhere with our advanced technology.

  • site_directed_mutagenesis_3

    Competitive Prices: Starting from $99 per mutant.

SERVICE DESCRIPTIONS

Enhance your research efficiency by partnering with us for seamless mutagenesis and gene synthesis solutions.

Here's what we need for your custom DNA template:

· DNA Template: Ship us your DNA template plasmid or bacterial culture. We recommend 4 μg of template DNA for optimal results.

· Sequence Details: Provide the complete sequence of the template, including the target gene and its vector.

· Mutation Specifications: Clearly outline your mutation requirements.

· Maps and Antibiotic Resistance: Include maps and antibiotic resistance details of both the template and destination vectors.

SERVICE PROCEDURE

  • Materials_provided_by_customer viral_delivery_left

    Materials provided
    by customer

  • QC_identification viral_delivery_left

    QC identification

  • Design_and_optimize_the_primer viral_delivery_left

    Design and
    optimize
    the primer

  • Cloned_into_original_vector_or_appointed_vector viral_delivery_left

    Cloned into original
    vector or appointed vector

  • Sequencing_&_Validation viral_delivery_left

    Sequencing
    & Validation

  • Delivery

    Delivery

SERVICE OPTIONS

Length Mutated Site EDT Price
< 3000 bp One 5-8 Business Days $99/mutation
Two 10-14 Business Days $149/mutation
Three 15-20 Business Days $199/mutation
3000 bp-8000 bp One 5-8 Business Days $129/mutation
Two 10-14 Business Days $179/mutation
Three 15-20 Business Days $229/mutation
> 8000 bp One Inquiry Inquiry
Two Inquiry Inquiry
Three Inquiry Inquiry

One mutation is defined as any combination of base changes within a 30-base frame.

STANDARD DELIVERABLES

  • 1tube_of_5_μg

    2-5μg of lyophilized plasmid containing target gene

  • Sequence_chromatograms

    Sequence chromatograms covering your gene (electronic)

  • Construct_map_for_the_plasmid

    Construct map for the plasmid (electronic)

  • Quality_assurance_certificate

    Quality assurance certificate

Request A Quote

Online Request Submission

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