Site-directed mutagenesis introduces changes to DNA fragments by PCR mutagenesis, including deletion, insertion and point mutation, to enable studies of gene regulation, DNA-protein interactions, protein structure/function, enzyme active sites and novel protein. Maximize your time for research,simply design your mutations and leave the rest to us. With Gene Universal, expect 100% accuracy in your mutant constructs, empowering your discoveries.
Features
● 100% Accuracy: Sequence-verified mutant constructs via Sanger sequencing.
● Unlimited Sites: Introduce mutations anywhere with our advanced technology.
● Competitive Prices: Starting from $99 per mutant.
Service Descriptions
Enhance your research efficiency by partnering with us for seamless mutagenesis and gene synthesis solutions.
Here's what we need for your custom DNA template:
● DNA Template: Ship us your DNA template plasmid or bacterial culture. We recommend 4 μg of template DNA for optimal results.
● Sequence Details: Provide the complete sequence of the template, including the target gene and its vector.
● Mutation Specifications: Clearly outline your mutation requirements.
● Maps and Antibiotic Resistance: Include maps and antibiotic resistance details of both the template and destination vectors.
Service Procedure
Materials provided
by customer
QC identification
Design and optimize
the primer
Cloned into original
vector or appointed vector
Sequencing & Validation
Delivery
Service Options
Length | Mutated Site | EDT | Price |
---|---|---|---|
< 3000 bp | One | 5-8 Business Days | $99/mutation |
Two | 10-14 Business Days | $149/mutation | |
Three | 15-20 Business Days | $199/mutation | |
3000 bp-8000 bp | One | 5-8 Business Days | $129/mutation |
Two | 10-14 Business Days | $179/mutation | |
Three | 15-20 Business Days | $229/mutation | |
> 8000 bp | One | Inquiry | Inquire |
Two | Inquiry | Inquire | |
Three | Inquiry | Inquire |
One mutation is defined as any combination of base changes within a 30-base frame.
Standard Deliverables
- 1tube of 5 μg of lyophilized plasmid containing target gene.
- Sequence chromatograms covering your gene (electronic).
- Construct map for the plasmid (electronic).
- Quality assurance certificate.
How to Order?