Bacterial Expression
  • Overview
  • Guaranteed Packages
  • Custom Package
  • Service Procedure

Gene Universal specializes in E.coli protein expression service with risk-free guarantee. Our Guaranteed Bacterial Recombinant Protein Expression Service Package consists of codon optimization service, followed by gene synthesis and subcloning, all the way through protein expression and purification. With the risk-free guarantee, no cost will be charged if the project fails. The overall success rate is over 95% in E.coli expression systems. Place your order today to experience unprecedented, high quality services yet competitive pricing Bacterial Protein Expression Services.

Guaranteed Packages

Risk-free E.coli Protein
Service Packages
Description
Amount
Timeline
Purity
Pricing
Risk-free Guaranteed Packages
(Tagged:His/MBP/GST)
‐Gene Synthesis & cloning
‐Expression pilot study
‐Scale-up and purification
‐Tag removal(optional)
‐Guaranteed quantity of purified protein
‐QC:SDS-PAGE and Western Blot(Tagged)
3mg(Guaranteed)
4-6 weeks
(From gene synthesis
to purified proteins)
85%
US$1,450
90%
US$1,850
95%
US$2,250
5mg(Guaranteed)
85%
US$1,950
90%
US$2,350
95%
US$2,750
10mg(Guaranteed)
85%
US$2,450
90%
US$2,850
95%
US$3,250
Other Quantity
Quote
Risk-free Guaranteed Packages
(Tag-free)
3mg(Guaranteed)
85%
US$2,850
90%
US$3,850
5mg(Guaranteed)
85%
US$3,950
90%
US$4,950
10mg(Guaranteed)
85%
US$6,050
90%
US$7,050
Other Quantity
Quote

NOTE:
  • 1. Risk-free for Guaranteed package: Quantity & Purity guaranteed,or it is FREE.

    2. Pricing includes gene synthesis <3000 bp.

    3. Pricing does not include endotoxin removal or some specific requirements. Additional charges apply for these services.

    4. Protein solubility is guaranteed but we DO NOT guarantee the
    bioactivity or functionality. If the protein expresses as inclusion bodies, refolding will be attempted to make the protein soluble.

    5. Protein is delivered in lyophilized powder whenever possible. If the solubility of protein changes after lyophilization, it will be shipped in liquid form.

  • 6. For tagged packages, we may use affinity tags to purify a protein and the final protein would be tag containing. For tag-free packages, we can provide service start with tag-free protein expression, and purified by SEC and ion exchange to ensure proper native folding, or start with fusion tag expression, and purified by tag removal, HIC, SEC or IEX to ensure proper native folding.

    7. Protein purity is determined by SDS-PAGE, and quantity is determined by Bradford/BCA/A280 assay.Purity determined by HPLC is also available, please inquire for specific requirements.

Custom Package (Charged by steps)

Steps
Specification
Timeline
Price
Deliverables
1.Gene synthesis or Subcloning,
Plasmid Prep
A).Gene synthesis sub cloning into expression vector
Varies
(Please inquire)
$0.15/bp (min:$79/gene)

‐Weekly progress reports

‐Desired quantity of purified,
soluble protein

‐Plasmid(synthesized by us,2-5ug)

‐QC data

B).Or provide your expression constructs, we’ll sub-clone your gene into our expression vector
$99/construct
2.Expression Pilot Study
Protein expression evaluation and optimization
$199
3.Protein Purification
Optimize and find the purification conditions of your protein (purity>85%)
$699
4.Scale-up and purification
Large scale expression & purification at your desired quantity (mg to g)
Quote
5.Tag removal and endotoxin removal and other steps(Optional)
Perform tag removal ,endotoxin removal,higher purity and other steps as needed per your request
Quote
6.QC testing
SDS-PAGE and Western Blot(tagged protein only)

Service Procedure

How to Order?

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