Gene Universal

Experience unparalleled reliability with Gene Universal's E. coli protein expression service, backed by our risk-free guarantee. Our Guaranteed Bacterial Recombinant Protein Expression Service Package encompasses codon optimization, gene synthesis, subcloning, and comprehensive protein expression and purification. With our risk-free guarantee, there's no charge if the project fails. Benefit from our exceptional success rate of over 95% in E. coli expression systems. Place your order today to access top-tier services at competitive pricing in Bacterial Protein Expression.

Service Procedure

Gene Synthesis

& Cloning
Protein expression evaluation

and optimization
Protein purification

pilot study
Scale-up with required

Quantity and Purity
QC & Shipping

Guaranteed Packages

Risk-free E.coli Protein Service Packages	Description	Amount	Timeline	Purity	Pricing
Risk-free Guaranteed Packages (Tagged:His/MBP/GST)	‐Gene Synthesis & cloning ‐Expression pilot study ‐Scale-up and purification ‐Tag removal(optional) ‐Guaranteed quantity of purified protein ‐QC:SDS-PAGE and Western Blot(Tagged)	3mg(Guaranteed)	4-6 weeks (From gene synthesis to purified proteins)	85%	US$1,450
				90%	US$1,850
				95%	US$2,250
		5mg(Guaranteed)		85%	US$1,950
				90%	US$2,350
				95%	US$2,750
		10mg(Guaranteed)		85%	US$2,450
				90%	US$2,850
				95%	US$3,250
		Other Quantity		Quote
Risk-free Guaranteed Packages (Tag-free)		3mg(Guaranteed)		85%	US$2,850
		3mg(Guaranteed)		90%	US$3,850
		5mg(Guaranteed)		85%	US$3,950
		5mg(Guaranteed)		90%	US$4,950
		10mg(Guaranteed)		85%	US$6,050
		10mg(Guaranteed)		90%	US$7,050
		Other Quantity		Quote

NOTE:

1. Risk-Free Guarantee: Quantity & Purity guaranteed, or it's FREE.

2. Inclusive Pricing: Gene synthesis <3000 bp included.

3. Protein Solubility: We guarantee solubility but not bioactivity or functionality. Inclusion bodies will be refolded for solubility.

4. Protein Solubility: We guarantee solubility but not bioactivity or functionality. Inclusion bodies will be refolded for solubility.

5. Additional Services: Endotoxin removal and specific requirements may require additional charges.
6. Quality Assurance: Purity is determined by SDS-PAGE, quantity by Bradford/BCA/A280assays. HPLC available upon request.

7. Delivery: Lyophilized powder preferred, liquid if solubility changes.

Custom Package (Charged by steps)

Steps	Specification	Timeline	Price	Deliverables
1.Gene synthesis or Subcloning, Plasmid Prep	A).Gene synthesis sub cloning into expression vector	Varies (Please inquire)	$0.15/bp (min:$79/gene)	‐Weekly progress reports ‐Desired quantity of purified, soluble protein ‐Plasmid(synthesized by us,2-5ug) ‐QC data
1.Gene synthesis or Subcloning, Plasmid Prep	B).Or provide your expression constructs, we’ll sub-clone your gene into our expression vector		$99/construct
2.Expression Pilot Study	Protein expression evaluation and optimization		$199
3.Protein Purification	Optimize and find the purification conditions of your protein (purity>85%)		$699
4.Scale-up and purification	Large scale expression & purification at your desired quantity (mg to g)		Quote
5.Tag removal and endotoxin removal and other steps(Optional)	Perform tag removal ,endotoxin removal,higher purity and other steps as needed per your request		Quote
6.QC testing	SDS-PAGE and Western Blot(tagged protein only)

Case Study

Case #1: Interleukin

Challenges

●Poor Solubility
●Tag-free, Affinity Purification is not Applicable
●Easy Polymerization
●Intrachain Disulfide Bond

Process Development

●Strain screening to promote disulfide bond formation in the cytoplasm
●Medium optimization, feeding strategy, especially harvest timing
●IEX Capture
●SEC Purification
●Buffer Exchange

QC Data:

M:Protein Marker

R: Reducing

NR:Non-Reducing
Yield: 350 mg/L, SDS-PAGE > 95%, SEC-HPLC > 95%

Case #2: Somatotropin

Challenges

●Inclusion Body Expression& Purification
●Disulfide Bond
●Easy Polymerization
●Unfolding & Refolding

Process Development

●Medium optimization, fermentation process control
●Dilution Refodling: dilution method/factors/speed
●Screening denaturants, stabilizers, aggregation inhibitors, pH, oxidant, reductant
●HIC purificatin, salt gradient elution

QC Data: