Yeast Expression System
  • Overview
  • Service Descriptions
  • Service Procedure
  • Service Options

Yeast expression is the most economical eukaryotic expression system for scale up production of both secreted and intracellular proteins that require post-translational modifications, including humanized antibodies. Gene Universal offers both Pichia Pastoris and Saccharomyces Cerevisiae expression services.

Service Descriptions

● Our Yeast Protein Expression Services start with Gene Universals’codon optimization service, followed by gene synthesis and sub cloning, all the way through protein expression and purification using our yeast protein expression system.

● Gene Universal’s yeast fermentation and expression services utilize a proprietary yeast fermentation technology for high-yield and high quality recombinant protein production in yeast .

● Gene Universal possesses the most advanced AKTA purifier system, along with the pre-positioned Ion column, molecular sieve, hydrophobic interaction column and affinity column.

Service Procedure

Yeast_Expression_System

Service Options

Yeast system service type:

No. Service type Working day Comment
1 Gene synthesis and subcloning 10-15 pPIC9K, pPICZalphaA….
2

Electrotransformation and PCR evaluation

7 GS115, X33, KM71…..  PCR screen 20 cloning
3 Expression test 7 10 cloning expression test
4 Small scale 10-12 200ml expression and purification
5 Large scale Enquiry Shaker flask/ Fermenter

Terms and Conditions

1. We will start the project according to your choice and will only charge the cost step by step.

2. Affinity purification, ion exchange purification, hydrophobic purification and SEC purification can be used. The expression system will affect the final protein purity and activity;

How to Order?

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