Insect Cell Expression
  • Overview
  • Service Descriptions
  • Service Procedure
  • Service Options

Compared with Bacterial Protein Expression ,there are several advantages of using baculovirus expression system, such as improved solubility, ability to incorporate post-translational modifications and higher yields for secreted proteins. Using our baculovirus expression system,we can establish the best possible protocol for your custom project and then scale up to get the highest expression of your target protein. With the stable experimental system, high expression level and post-translation modification, baculovirus expression is widely recognized as a powerful tool for expressing large-scale proteins.

Service Descriptions

● Our baculovirus expression Services start with Gene Universals’codon optimization service, followed by gene synthesis and sub cloning, all the way through protein expression and purification using our bacterial protein expression system.

● Gene Universal possesses the most advanced AKTA purifier system, along with the pre-positioned Ion column, molecular sieve, hydrophobic interaction column and affinity column.

● Gene Universal’s Endotoxin Removal Service can effectively reduce the endotoxin level to under 10 EU/mg during recombinant protein production with a success rate of 98%.

Service Procedure

Insect_Cell_Expression

Service Options

Bac-to-Bac system service type

No. Service type Working day Comment
1 Gene synthesis and subcloning 10-15 pFastBac1, pFastBacHT….
2

Bacmid preparation ,transfection and expression evaluation

14 PCR evaluation and P1 virus preparation
3 P2 virus amplification 6 P2 virus amplification and MOI optimization
4 Small scale 8 200ml expression and purification
5 Large scale Enquiry Shaker flask/ bioreactor

Terms and Conditions

1. We will start the project according to your choice and will only charge the cost step by step.

2. Affinity purification, ion exchange purification, hydrophobic purification and SEC purification can be used. The expression system will affect the final protein purity and activity.

How to Order?

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