The mammalian cell expression system, known for its intricate glycosylation processes, is essential for achieving precise post-translational modifications and correct protein folding. Particularly crucial for downstream applications, transient expression in mammalian cells is widely utilized in the development and production of recombinant protein drugs, notably therapeutic recombinant monoclonal antibodies.
Gene Universal's mammalian expression services streamline the production and delivery of high-quality proteins and recombinant antibodies (rAbs), ranging from microgram to gram quantities, in CHO or HEK293 cells. With guaranteed protein quantity and purity, we leverage proprietary transient mammalian expression technology to optimize cellular pathways, resulting in exceptional protein and antibody yields (up to 3 g/L).
Our guaranteed mammalian protein expression packages encompass gene synthesis and subcloning, progressing seamlessly through protein expression and purification using our proprietary mammalian protein expression and purification technologies. Trust Gene Universal to propel your research forward with reliable and efficient mammalian protein expression solutions.
Service Descriptions
● One-Stop Solution: Seamless integration from gene synthesis to protein and antibody production.
● Inclusive Gene Synthesis: No additional cost for gene synthesis.
● Competitive Pricing: Cost-effective solutions tailored to your scaling needs.
● Swift Turnaround: Expedited service with turnaround times as fast as 2 weeks.
● Ultra-Low Endotoxin Levels: as low as 1 EU/mg.
● No Setup Fees: Transparent pricing without setup fees.
● Flexible Packages: Customize your package according to your specific requirements.
Service Procedure
Protein
Sequence
Gene Synthesis
Plasmid Prep
Transient Transfection
& Expression
Purification & QC
Fast
Delivery
Service Options
Mammalian system service type
Service Step | Service Description | Timeline | Deliverables |
---|---|---|---|
Gene Synthesis |
● Codon Optimization & Gene Synthesis ● Subcloning ● Plasmid Preparation |
2 to 3 weeks |
● Expression Plasmid Contain GOI ● Purified Recombinant Protein (SDS-PAGE & SEC-HPLC Detection) ● COA Report |
Pilot Study |
● Pilot Protein Expression ● Pilot Protein Purification ● QC Analysis |
||
Scale-up Production |
● Large-scale Protein Expression ● Purification ● QC Analysis |
3 to 4 weeks |
● Purified Recombinant Protein (SDS-PAGE & SEC-HPLC Detection) ● COA Report |
Terms and Conditions
1. Flexibility in Payment: Begin your project according to your preferences, and we'll charge only for each step as it progresses, ensuring transparency and flexibility.
2. Diverse Purification Methods: Choose from affinity purification, ion exchange purification, hydrophobic purification, and SEC purification. Keep in mind that the expression system selected will impact the final protein's purity and activity, ensuring optimal outcomes tailored to your needs.
● Fibroblast growth factor 21 with N-Fc tag (293F)
M: Protein Marker
Lane 1: Reducing
Lane 2: Non-Reducing
SDS-PAGE > 95%, SEC-HPLC > 95%
● Scfv with His tag (CHO-S)
M: Protein Marker
Lane 1: Reducing
Lane 2: Non-Reducing
SDS-PAGE > 95%, SEC-HPLC > 95%
How to Order?