Single-Stranded DNA Synthesis
  • Overview
  • Service Features
  • Service Details
  • Applications Of ssDNA Synthesis

Gene Universal now offers high-quality, sequence-verified single-stranded DNA (ssDNA), ideal for maximizing the editing efficiency of your CRISPR-mediated gene knock-in, in-vitro transcription, and other experiments. With high editing efficiency and lower off-target integration, ssDNA is considered the optimal CRISPR Homology Directed Repair (HDR) template for creating gene knock-ins to develop transgenic animal models and cell lines.

Advantages of leveraging ssDNA as CRISPR-mediated gene knock-in HDR Templates.

Lower cellular toxicity

Reduced off-target integration

High efficiency

Increased editing accuracy

Service Features

● Sanger sequencing verifies sequence accuracy.

● Non-detectable levels of dsDNA and minimal DNA base damage.

● Free gene template storage, supporting future reorders.

● Expertise in synthesizing difficult genes as ssDNA templates.

● Industry-leading turnaround for rapid delivery.

● Yield flexibility: Choose from 3, 5, 10, or more µg of lyophilized fragments.

Service Details

Length (Nucleotides) Quantity Price Turnaround Time (Business Days)
151-500 3 ug $350 10-12 Business Days
5 ug $500
10 ug $750
>10 ug Inquiry Inquiry
501-3000 3 ug $0.75/nt 15-18 Business Days
5 ug $0.9/nt
10 ug $1.2/nt
>10 ug Inquiry Inquiry
3001-5000 Inquiry Inquiry Inquiry

* Please note: Shipped as lyophilized powder. Enjoy 50% off complementary sequence for every order. For complex sequences (GC/AT rich, poly structure, hairpin, repeats), please request a quote.

Applications Of ssDNA Synthesis

1. CRISPR-mediated gene knock-in, replacement, or correction

Double Stranded Breaks (DSBs)

Guide RNA (gRNA)

Protospacer Adjacent Motif (PAM)

Non-Homologous End-joining (NHEJ)

Homology Directed Repair (HDR)

2. In vitro transcription

Transcription initiates DNA-based gene expression, converting DNA segments into RNA, particularly mRNA, through RNA polymerase enzymes. In vitro transcription (IVT) utilizes RNA polymerases (T7, T3, or SP6) and DNA templates to transcribe RNA molecules. Templates for IVT include double-stranded DNA fragments, circular plasmid DNA, and single-stranded donor oligonucleotides (ssODN) or single-stranded single-stranded DNA (ssDNA).

Other application areas

Antibody Discovery

Cancer Biology


Food technology

Deliverables and Quality Control Documents

● Delivery format: Lyophilized

● Digested by S1 nuclease, dsDNA residues will be verified by agarose gel electrophoresis

● Sequence verified via Sanger sequencing

● Purity tested by gel -electrophoresis, sterilized by 0.22um filter membrane, A260/A280=1.8-2.0

How to Order?


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