Single-Stranded DNA Synthesis
  • Overview
  • Service Features
  • Service Details
  • Applications Of ssDNA Synthesis

Single-stranded DNA (ssDNA), with high editing efficiency and lower off-target integration, is thought to be the best CRISPR Homology Directed Repair (HDR) template for creating gene knock-in to develop transgenic animal models and cell lines. Gene Universal now offers high quality and sequence verified ssDNA for maximizing the editing efficiency of your CRISPR-mediated gene knock-in, in-vitro transcription, and more experiments.

Advantages of leveraging ssDNA as CRISPR-mediated gene knock-in HDR Templates.

Lower cellular toxicity

Reduced off-target integration

High efficiency

Increased editing accuracy

Service Features

● Sequence verification by sanger sequencing

● Non-detectable levels of dsDNA and minimum DNA base damage

● Free gene template storage, supporting further re-orders

● Expertise in synthesizing difficult genes as ssDNA templates

● Rapid delivery – Industry-leading turnaround time

● Yield flexibility – Choose 3, 5, 10 or even more µg of lyophilized fragments

Service Details

Length (Nucleotides) Quantity Price Turnaround Time (Business Days)
151-500 3 ug $350 10-12 Business Days
5 ug $500
10 ug $750
>10 ug Inquiry Inquiry
501-3000 3 ug $0.75/nt 15-18 Business Days
5 ug $0.9/nt
10 ug $1.2/nt
>10 ug Inquiry Inquiry
3001-5000 Inquiry Inquiry Inquiry

* Please note: Shipped as lyophilized powder, 50% off complementary sequence for every order, complex sequences (GC/AT rich, poly structure, hairpin, repeats) need to be quoted.

Applications Of ssDNA Synthesis

1. CRISPR-mediated gene knock-in, replacement, or correction

Double Stranded Breaks (DSBs)

Guide RNA (gRNA)

Protospacer Adjacent Motif (PAM)

Non-Homologous End-joining (NHEJ)

Homology Directed Repair (HDR)

2. In vitro transcription

Transcription is the first of several steps of DNA based gene expression in which a particular segment of DNA is copied into RNA (especially mRNA) by the enzyme RNA polymerase. In vitro transcription (IVT) method means the transcription of RNA molecules, via RNA polymerase (T7, T3, or SP6) by using DNA templates in vitro. Several types of DNA templates could be used for IVT, including double-stranded DNA fragments, circular plasmid DNA, and single-stranded donor oligonucleotides (ssODN) or single-stranded single-stranded DNA (ssDNA).

Other application areas

Antibody Discovery

Cancer Biology

Biofuels

Food technology

Deliverables and Quality Control Documents

● Delivery format: Lyophilized

● Digested by S1 nuclease, dsDNA residues will be verified by agarose gel electrophoresis

● Sequence verified via Sanger sequencing

● Purity tested by gel -electrophoresis, sterilized by 0.22um filter membrane, A260/A280=1.8-2.0

How to Order?

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